Background: Parkinson disease (PD) is a neurodegenerative disorder characterized by progressive degeneration of the dopaminergic neurons in the substantia nigra, presumably due to increased apoptosis. In previous studies, we showed altered expression of proteins involved in mammalian target of rapamycin (mTOR) antiapoptotic and double-stranded RNA-dependent protein kinase (PKR) apoptotic pathways of translational control in experimental cellular and animal models of PD. Results: In this work, our results showed clear modifications in the expression of kinases involved in mTOR and PKR apoptosis pathways, in lymphocytes of PD patients treated or not with anti-PD treatment (levodopa), which confirmed the role played by apoptosis in the pathogenesis of this disease and the positive effect of treatment with medication on this parameter. Others proteins involved in apoptosis were also evaluated in lymphocytes of patients as the expression of the peripheral benzodiazepine receptor and caspase-3. Conclusion: Translational control is altered in PD and hence its evaluation in peripheral blood mononuclear cells may serve as an early marker of apoptosis and indicate the efficacy of the dopaminergic treatment.

中文翻译：

---

帕金森患者淋巴细胞蛋白合成和凋亡的调控：多巴胺能治疗的效果

背景：帕金森病 (PD) 是一种神经退行性疾病，其特征是黑质中多巴胺能神经元的进行性变性，可能是由于细胞凋亡增加所致。在以前的研究中，我们在 PD 的实验细胞和动物模型中显示了参与哺乳动物雷帕霉素靶标 (mTOR) 抗凋亡和双链 RNA 依赖性蛋白激酶 (PKR) 翻译控制凋亡途径的蛋白质表达的改变。结果：在这项工作中，我们的结果显示，在接受或未接受抗 PD 治疗（左旋多巴）治疗或未接受抗 PD 治疗（左旋多巴）的 PD 患者的淋巴细胞中，参与 mTOR 和 PKR 凋亡途径的激酶的表达发生了明显的改变，这证实了凋亡在这种疾病的发病机制以及药物治疗对该参数的积极影响。其他参与细胞凋亡的蛋白质也在患者淋巴细胞中评估为外周苯二氮卓受体和 caspase-3 的表达。结论：翻译控制在 PD 中发生改变，因此其在外周血单核细胞中的评估可作为细胞凋亡的早期标志物，并表明多巴胺能治疗的疗效。