Canine adenovirus (CAV) circulates as two distinct serotypes, CAV-1 and CAV-2, which are antigenically related but differ in their clinical manifestations. CAV is one of the important viral agents in the etiology of canine gastroenteritis. Here, we report the molecular surveillance and genetic characterization of CAV from clinical cases of canine gastroenteritis. A total of 302 fecal/rectal swabs were collected from dogs presented with gastroenteritis at various clinics in and around Hyderabad, India during 2018–19. These samples were tested for CAV using polymerase chain reaction with primers designed for the CAV E3 gene and the virus was isolated from positive samples. CAV-2 nucleic acid was present in 4.9% of the test samples. The partial sequence analyses of the E3 gene of the CAV-2 isolates revealed a frameshift mutation by insertion of nucleotide “G” at 1077 position of E3 gene, which resulted in an extension of the polypeptide chain by eleven amino acids. As a result, isolates from the current study formed a novel group, and the virus that was previously subdivided into two groups worldwide is now categorized under three. The study identifies a novel group of CAV-2 circulating in India providing an updated information regarding CAV-2.
Intervirology

中文翻译：

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从印度犬胃肠炎病例中分离出的犬腺病毒2的E3基因基础遗传特征揭示了该病毒的新类别。

犬腺病毒（CAV）以两种不同的血清型传播，CAV-1和CAV-2具有抗原相关性，但临床表现不同。在犬胃肠炎的病因学中，CAV是重要的病毒制剂之一。在这里，我们从犬胃肠炎的临床病例报告了CAV的分子监测和遗传特征。在2018-19期间，在印度海得拉巴及周边地区的各个诊所从患有肠胃炎的狗中总共收集了302支粪便/直肠拭子。使用聚合酶链反应和专为CAV E3基因设计的引物，对这些样品的CAV进行测试，并从阳性样品中分离出病毒。CAV-2核酸存在于4.9％的测试样品中。E3的部分序列分析通过在E3基因的1077位置插入核苷酸“ G”，CAV-2分离株的cDNA基因揭示了移码突变，这导致多肽链延长了11个氨基酸。结果，从当前研究中分离出的病毒形成了一个新的群体，以前在世界范围内细分为两类的病毒现在被归类为三类。该研究确定了在印度流行的一组新的CAV-2，提供了有关CAV-2的最新信息。
病毒学