Ureteropelvic junction (UPJ) obstruction is a common problem in children, but its etiology remains unclear. In this study, the proteome profiles of the obstructed segment and its surrounding distal and proximal parts were comparatively evaluated. Twelve children younger than 2 years of age with unilateral intrinsic UPJ obstruction were included. The excised operational tissue was divided into three parts immediately after resection: the obstructed part (Obst), the distal normal ureteral part (Dist), and the proximal part of the obstructed segment (Prox). Proteins extracted from the tissue samples were subjected to two-dimensional gel electrophoresis analysis to identify differentially regulated proteins. Spot analysis revealed that four proteins, namely tropomyosin beta and alpha-1 chains, actin and desmin, were upregulated in Obst in comparison to Dist. A similar analysis between Obst and Prox showed that heat shock protein beta-1 and carbonic anhydrase-1 were upregulated in Obst, while tropomyosin alpha 3 chain and ATP synthase beta were upregulated in Prox. The last comparative analysis between Dist and Prox revealed upregulation of annexin-A5 and annexin-A1 in Dist and vimentin, mitochondrial ATP synthase subunit-beta, peroxiredoxin-2, and apolipoprotein-A1 in Prox. Bioinformatics analysis using the STRING server indicated that the differentially regulated proteins, altogether, point to the changes occurring in muscle filament sliding pathway. When regulations occurring in each group were mutually compared, a change in lipase inhibition activity was detected by STRING. This is the first study scrutinizing changes occurring in protein profiles in UPJ.

中文翻译：

---

输尿管骨盆连接处梗阻及输尿管周围组织的蛋白质组比较分析

输尿管骨盆连接部 (UPJ) 梗阻是儿童常见的问题，但其病因尚不清楚。在这项研究中，对阻塞段及其周围远端和近端部分的蛋白质组谱进行了比较评估。包括 12 名 2 岁以下患有单侧内在 UPJ 梗阻的儿童。切除后立即将切除的手术组织分为三部分：阻塞部分（Obst）、远端正常输尿管部分（Dist）和阻塞部分的近端部分（Prox）。从组织样本中提取的蛋白质进行二维凝胶电泳分析，以确定差异调节的蛋白质。斑点分析显示四种蛋白质，即原肌球蛋白β和α-1链、肌动蛋白和结蛋白，与 Dist 相比，Obst 上调。Obst 和 Prox 之间的类似分析表明，热休克蛋白 beta-1 和碳酸酐酶-1 在 Obst 中上调，而原肌球蛋白 α 3 链和 ATP 合酶 beta 在 Prox 中上调。Dist 和 Prox 之间的最后比较分析显示，Dist 中的膜联蛋白-A5 和膜联蛋白-A1 上调，而 Prox 中的波形蛋白、线粒体 ATP 合酶亚基-β、过氧化物酶-2 和载脂蛋白-A1 上调。使用 STRING 服务器的生物信息学分析表明，差异调节的蛋白质共同指向肌肉丝滑动途径中发生的变化。当相互比较各组中发生的规律时，STRING检测到脂肪酶抑制活性的变化。