Malnad Gidda is a dwarf indigenous cattle breed of India, which is known for its uniqueness of calving every year under a low input grazing system of rearing. Bulls of Malnad Gidda are known to be highly fertile even in stress conditions. However, the proteomic profiling of semen of this breed has not been investigated so far, which might provide a platform for a better understanding of its semen quality and male fertility. Therefore, we made an effort to characterize and quantify the proteome of seminal plasma and spermatozoa components of Malnad Gidda semen using a high-resolution mass spectrometry platform. We identified 2814 proteins from spermatozoa and 1974 proteins from the seminal plasma of this breed. Furthermore, >90% of proteins from each fraction were quantified using the intensity-based absolute quantification. We observed signal peptides in 33% of seminal plasma proteins, indicating their secretory nature. Gene Ontology analysis revealed their involvement in cytoskeletal assembly associated with sperm head, sperm motility, acrosome reaction, seminal plasma binding, and spermatogenesis-associated protein. An in-depth proteome profiling of semen of a unique indigenous cattle breed of India was carried out. Our findings could provide a reference for further studies on sperm functions, semen quality, and reproductive health of Bos indicus cattle. Mass spectrometry data generated in this study is deposited and publicly made available through ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD014172.

中文翻译：

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印度土著 Malnad Gidda (Bos indicus) 牛精液的深度蛋白质组分析。

Malnad Gidda 是印度一种矮小的本土牛品种，以其在低投入放牧系统下每年产犊的独特性而闻名。众所周知，即使在压力条件下，马尔纳德吉达的公牛也具有很高的繁殖力。然而，迄今为止尚未对该品种精液的蛋白质组学进行研究，这可能为更好地了解其精液质量和雄性生育能力提供一个平台。因此，我们努力使用高分辨率质谱平台对 Malnad Gidda 精液的精浆和精子成分的蛋白质组进行表征和量化。我们从该品种的精子中鉴定出 2814 种蛋白质，从精浆中鉴定出 1974 种蛋白质。此外，使用基于强度的绝对量化对来自每个部分的 >90% 的蛋白质进行量化。我们在 33% 的精浆蛋白中观察到信号肽，表明它们的分泌性质。基因本体分析显示它们参与了与精子头部、精子运动、顶体反应、精浆结合和精子发生相关蛋白相关的细胞骨架组装。对印度独特的本土牛品种的精液进行了深入的蛋白质组分析。我们的研究结果可为进一步研究精子功能、精液质量和生殖健康提供参考。对印度独特的本土牛品种的精液进行了深入的蛋白质组分析。我们的研究结果可为进一步研究精子功能、精液质量和生殖健康提供参考。对印度独特的本土牛品种的精液进行了深入的蛋白质组分析。我们的研究结果可为进一步研究精子功能、精液质量和生殖健康提供参考。Bos indicus牛。本研究中生成的质谱数据由 ProteomeXchange Consortium 通过 PRIDE 合作伙伴存储库存储并公开提供，数据集标识符为 PXD014172。