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Hippo signaling, actin polymerization, and follicle activation in fragmented human ovarian cortex.
Molecular Reproduction and Development ( IF 2.5 ) Pub Date : 2020-06-07 , DOI: 10.1002/mrd.23353
Stine A Lunding 1 , Anders N Andersen 1 , Lilja Hardardottir 1, 2 , Hanna Ø Olesen 2 , Stine G Kristensen 2 , Claus Y Andersen 2 , Susanne E Pors 2
Affiliation  

The Hippo pathway has been associated with regulation of early follicle growth. Studies of murine ovaries suggest that changes in the actin cytoskeleton, caused by fragmentation, result in inhibition of the Hippo pathway, and in turn, may activate follicle growth. In humans, the connections between fragmentation, the actin cytoskeleton, and follicle activation are yet to be confirmed. In this study, we investigated the impact in vitro fragmentation of a human ovarian cortex on (a) actin polymerization, (b) components of the Hippo pathway, and (c) follicle growth in vivo. The results showed that the ratio between globular and filamentous actin remained unchanged at all timepoints (0, 10, 30, 60, 120, and 240 min) following tissue fragmentation. Neither was the Hippo pathway effector protein YES‐associated protein upregulated nor was gene expression of the downstream growth factors CCN2 , CCN3 , or CCN5 increased at any timepoint in the fragmented cortex. Furthermore, the number of growing follicles was similar in fragmented and intact cortex pieces after 6 weeks' xenotransplantation. However, the total number of surviving follicles was considerably lower in the fragmented cortex compared with intact tissue, suggesting detrimental effects of fragmentation on tissue grafting. These results indicate that fragmentation is likely to be ineffective to activate follicle growth in the human ovarian cortex.

中文翻译:

破碎的人类卵巢皮质中的河马信号,肌动蛋白聚合和卵泡活化。

河马途径与早期卵泡生长的调节有关。鼠卵巢的研究表明,由碎片引起的肌动蛋白细胞骨架的变化会导致河马途径的抑制,进而激活卵泡的生长。在人类中,片段化,肌动蛋白细胞骨架和卵泡活化之间的联系尚待证实。在这项研究中,我们调查了人类卵巢皮质的体外破碎对(a)肌动蛋白聚合,(b)Hippo途径的成分和(c)体内卵泡生长的影响。结果显示,在组织破碎后的所有时间点(0、10、30、60、120和240分钟),球状肌动蛋白与丝状肌动蛋白的比例均保持不变。CCN2CCN3CCN5在碎片皮质中的任何时间点增加。此外,异种移植6周后,破碎且完整的皮质碎片中生长的卵泡数量相似。但是,与完整的组织相比,破碎的皮层中存活的卵泡总数要低得多,这表明破碎对组织移植的有害影响。这些结果表明,碎裂可能无法有效激活人卵巢皮质的卵泡生长。
更新日期:2020-06-28
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