The present study aimed to assess the annual reproductive cycle of female brown banded bamboo shark, Chiloscyllium punctatum, from the Persian Gulf using macroscopic and microscopic evaluation of reproductive tract. The annual cycle of gonadal steroids (17b-estradiol (E2), progesterone (P4) and testosterone (T)) was also assessed in this shark. Totally, 130 female C. punctatum were collected from the Bahrakan Creek (located at the northwest of the Persian Gulf) between January 2018 and March 2019. Females were oviparous with an external-type ovary and only one functional ovary. Five sexual maturity stages were recognized based on macroscopic and microscopic evaluation including Immature I (August-October), Immature II (November-January), Mature (February-March), Pregnant (April-May), and Spent (Jun-July). The structural changes in the oviducts, oviducal glands and uterus throughout the annual reproductive cycle were consistent with their roles in the egg movement, the egg capsule production, and sperm storage. The plasma levels of the gonadal steroids were associated with morphological changes in the reproductive tract. 17b-estradiol showed two detectable peaks during March (close to ovulation) and June (just before mating). Progesterone and testosterone displayed a peak just before ovulation. This article is protected by copyright. All rights reserved.

中文翻译：

---

来自波斯湾的雌性棕带竹鲨的年度生殖周期，斑纹竹鲨（Elasmobranchii：Hemiscylliidae）：生殖的形态学和内分泌相关性

本研究旨在通过生殖道的宏观和微观评估来评估来自波斯湾的雌性褐带竹鲨 Chiloscyllium punctatum 的年度生殖周期。还在这条鲨鱼中评估了性腺类固醇（17b-雌二醇（E2）、孕酮（P4）和睾酮（T））的年度周期。2018 年 1 月至 2019 年 3 月，在巴哈拉干河（位于波斯湾西北部）共采集了 130 只雌性点状线虫。雌性为卵生，外型卵巢，只有一个功能性卵巢。根据宏观和微观评估识别出五个性成熟阶段，包括未成熟 I（8 月至 10 月）、未成熟 II（11 月至 1 月）、成熟（2 月至 3 月）、怀孕（4 月至 5 月）和已花期（6 月至 7 月） . 输卵管的结构变化，在整个年度生殖周期中，输卵管腺​​体和子宫与它们在卵子运动、卵囊产生和精子储存中的作用是一致的。性腺类固醇的血浆水平与生殖道的形态变化有关。17b-雌二醇在 3 月（接近排卵期）和 6 月（就在交配前）显示出两个可检测的峰值。孕酮和睾酮在排卵前出现峰值。本文受版权保护。版权所有。17b-雌二醇在 3 月（接近排卵期）和 6 月（就在交配前）显示出两个可检测的峰值。孕酮和睾酮在排卵前出现峰值。本文受版权保护。版权所有。17b-雌二醇在 3 月（接近排卵期）和 6 月（就在交配前）显示出两个可检测的峰值。孕酮和睾酮在排卵前出现峰值。本文受版权保护。版权所有。