Alpinia officinarum Hance of the Chinese traditional herb for the treatment of emesis, abdominal pain and diarrhea has been used to counteract gastric disease induced by indomethacin in rats without obvious side effects. However, the role of herb-drug interaction between indomethacin and A. officinarum based on pharmacokinetic, tissue distribution and excretion still remains unknown. In this study, an ultra-fast liquid-tandem mass spectrometry (UFLC-MS/MS) method was developed for simultaneous determination of indomethacin and its three metabolites, O-desmethylindomethacin (ODI), deschlorobenzoylindomethacin (NDI) and indomethacin acyl-β-D-glucuronide (IDAβG) by oral administration of indomethacin solution with and without the ethanolic extract of A. officinarum and applied to comparative pharmacokinetic, tissue distribution and excretion studies. Our results clarified that oral administration of A. officinarum produced significant alterations in the pharmacokinetic parameters of indomethacin. And the pharmacokinetic interaction between indomethacin and A. officinarum reduced the systemic exposure of indomethacin and increased its elimination. Tissue distribution results demonstrated that co-administration of A. Officinarum could not reduce the accumulation of indomethacin in the target tissue of the stomach, but could accelerate the excretions of indomethacin and its three metabolites including ODI, NDI and IDAβG in the bile and feces of rats in the excretion study. Therefore, A. Officinarum might have a gastrointestinal protective effect through the interaction role with indomethacin based on the pharmacokinetics and excretion in rats.

中草药高良姜用于治疗呕吐，腹痛和腹泻已被用于抵消吲哚美辛引起的大鼠胃部疾病，而没有明显的副作用。然而，基于药代动力学，组织分布和排泄，吲哚美辛与厚朴曲霉之间药草相互作用的作用仍然未知。在这项研究中，开发了一种超快液相串联质谱（UFLC-MS / MS）方法，用于同时测定吲哚美辛及其三种代谢物O-去甲基吲哚美辛（ODI），去氯苯甲酰吲哚美辛（NDI）和吲哚美辛酰基-β- D-葡萄糖醛酸苷（IDAβG）通过吲哚美辛溶液的口服和不服用A. officinarum的乙醇提取物并用于比较药代动力学，组织分布和排泄研究。我们的结果表明，口服A.officinarum会导致吲哚美辛的药代动力学参数发生重大变化。吲哚美辛与厚朴曲霉之间的药代动力学相互作用减少了吲哚美辛的全身暴露并增加了其消除。组织分布结果表明，A。Officinarum的共同给药不能减少吲哚美辛在胃靶组织中的积累，但是可以促进吲哚美辛及其三种代谢产物（包括ODI，NDI和IDAβG）在胆汁和粪便中的排泄。大鼠进行排泄研究。因此，A。Officinarum 通过与吲哚美辛的相互作用，根据大鼠的药代动力学和排泄作用，可能具有胃肠道保护作用。