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Warburg’s method as a simple tool for measuring oxygen uptake in spray-dried emulsions
Food Structure ( IF 4.7 ) Pub Date : 2020-06-06 , DOI: 10.1016/j.foostr.2020.100143
Bruna Barbon Paulo , Fernando Divino de Oliveira Júnior , Izabela Dutra Alvim , Ana Silvia Prata

Lipid oxidation is usually responsible for oil downgrading of spray-dried powders and it is strongly dependent on the storage conditions. Direct measurements of the oxygen uptake using cost-effective methods combined with the study of water adsorption of the powders allow evaluating their barrier properties against oxidation. Therefore, a low-cost and simple apparatus based on the Warburg’s manometric technique was built, and its results were compared to Rancimat and Oxipres methods. Firstly, free unsaturated oil (fish oil) was evaluated and data showed a strong positive correlation between the oxygen uptakes for the new apparatus (0.015 mL O2/h) and Oxipres method (Pearson’s coefficient = 0.97). After, different surface-active compounds (SAC) (whey protein isolate – WPI, gelatin – GE, or modified starch – MS) mixed to maltodextrin (MD) were used to encapsulate the fish oil via spray drying. The lowest oxygen uptake was obtained by MD + WPI (0.0018 mL O2/h), followed by MD + GE (0.0103 mL O2/h) and MD + MS (0.0291 mL O2/h), with similar behavior obtained by Rancimat method. The lowest protective barrier of MD + MS-powders was confirmed by the comparatively higher susceptibility to water adsorption, and consequently the lower glass transition temperature (∼58.7 °C at water activity = 0.3−0.4). This study presented a successful creation of an apparatus to provide useful, fast and effective information about oxygen consumption by fish oil and encapsulated fish oil, allowing the understanding of the protection of SAC against oxidation in spray-dried emulsions. The proposed apparatus can be easily implemented in laboratories, with potential application for other oxygen-sensitive products.



中文翻译:

Warburg作为测量喷雾干燥乳液中氧气吸收的简单工具的方法

脂质氧化通常是造成喷雾干燥粉末油降级的原因,它强烈取决于储存条件。使用经济高效的方法直接测量氧气吸收量,并结合粉末的水吸附研究,可以评估粉末对氧化的阻隔性能。因此,建立了一种基于Warburg测压技术的低成本,简单的仪器,并将其结果与Rancimat和Oxipres方法进行了比较。首先,对游离的不饱和油(鱼油)进行了评估,数据表明,新设备(0.015 mL O 2)的摄氧量之间存在强正相关/ h)和Oxipres方法(皮尔森系数= 0.97)。之后,将不同的表面活性化合物(SAC)(乳清蛋白分离物-WPI,明胶-GE或改性淀粉-MS)与麦芽糖糊精(MD)混合使用,通过喷雾干燥将鱼油包封。通过MD + WPI(0.0018 mL O 2 / h),然后是MD + GE(0.0103 mL O 2 / h)和MD + MS(0.0291 mL O 2)获得最低的氧气吸收量/ h),具有通过Rancimat方法获得的类似行为。MD + MS粉末的最低防护层是通过相对较高的吸水敏感性确定的,因此玻璃化转变温度较低(在水活度= 0.3-0.4时约为58.7°C)。这项研究成功地创造了一种设备,可提供有关鱼油和胶囊化鱼油耗氧量的有用,快速和有效的信息,从而使您能够了解SAC在喷雾干燥的乳剂中免受氧化的保护作用。所提出的装置可以容易地在实验室中实施,并可能用于其他对氧气敏感的产品。

更新日期:2020-06-06
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