Food Bioscience ( IF 5.2 ) Pub Date : 2020-06-07 , DOI: 10.1016/j.fbio.2020.100670 Soufian Ider , Yanath Belguesmia , Guillaume Cazals , Rabah Boukherroub , Françoise Coucheney , Mabrouk Kihal , Christine Enjalbal , Djamel Drider
Six Trichosporon asahii strains designated ICVY021, ICVY022, ICVY023, ICVY024, ICVY025 and ICVY026 were isolated from camel milk and studied for their inhibitory activities against a large number of fungi and bacteria as target organisms. Only strain ICVY021 was able to inhibit Kocuria rhizophila CIP 53.45, through production of an extracellular heat-stable, proteinaceous antibacterial peptide, with partial amino acid sequences of PPFPK and CTHV(L/I)(K/Q) or TCHV(L/I)(K/Q), determined using LC/MS/MS. This peptide, named oranicin P16, was thought to impede the cell-division mechanism. The anti- K. rhizophila activity was confirmed, in situ, using skim milk as a food matrix. T. asahii ICVY021 did not show any hemolytic activity or any cytotoxicity effect against eukaryotic Caco-2 cells. T. asahii ICVY021 was sensitive to azoles antifungal compounds, but not to 5-fluorocytosine and anidulafungin.
中文翻译:
骆驼奶中发现的从Trichosporon asahii ICVY021中分离出的抗菌肽Oranicin P16抑制了Kocuria rhizophila
从骆驼奶中分离了六种命名为ICVY021,ICVY022,ICVY023,ICVY024,ICVY025和ICVY026的Trichosporon asahii菌株,并研究了它们对作为目标生物的大量真菌和细菌的抑制活性。只有菌株ICVY021能够通过产生胞外热稳定的蛋白质抗菌肽(具有PPFPK和CTHV(L / I)(K / Q)或TCHV(L / I)的部分氨基酸序列)来抑制嗜酸Kocuria CIP 53.45。 (K / Q),使用LC / MS / MS确定。有人认为这种名为奥拉辛素P16的肽会阻碍细胞分裂机制。使用脱脂乳作为食物基质原位证实了其抗根瘤菌的活性。旭ICVY021对真核Caco-2细胞未显示任何溶血活性或任何细胞毒性作用。T. asahii ICVY021对唑类抗真菌化合物敏感,但对5-氟胞嘧啶和阿尼芬净不敏感。