Here, we provide the first mass molecular screening of medically important mosquitoes for Bartonella species using multiple genetic markers. We examined a total of 72,115 mosquito specimens, morphologically attributed to Aedes vexans (61,050 individuals), Culex pipiens (10,484 individuals) and species of the Anopheles maculipennis complex (581 individuals) for Bartonella spp. The initial screening yielded 63 Bartonella-positive A. vexans mosquitoes (mean prevalence 0.1%), 34 Bartonella-positive C. pipiens mosquitoes (mean prevalence 0.3%) and 158 Bartonella-positive A. maculipennis group mosquitoes (mean prevalence 27.2%). Several different Bartonella ITS sequences were recovered. This study highlights the need for molecular screening of mosquitoes, the most important vectors of arthropod-borne pathogens, for potential bacterial agents.

在这里，我们提供了使用多种遗传标记物对巴尔通体物种具有重要医学意义的蚊子的首次大规模分子筛选。我们检查了总共72,115蚊子的标本，其形态学上归因于白纹伊蚊（61,050个体），淡色库蚊（10,484个体）和Bartonella spp的按蚊群（581个体）。初步筛选产生了63株巴尔通体阳性的墨西哥州立蚊（平均患病率0.1％），34株巴尔通体呈阳性的C. pipiens蚊子（平均患病率0.3％）和158巴尔通体阳性A. maculipennis群蚊（平均患病率为27.2％）。回收了几种不同的Bartonella ITS序列。这项研究强调需要分子筛查蚊子（节肢动物传播的病原体最重要的媒介）以寻找潜在的细菌。