Salmonella, one of the most common foodborne pathogens, poses a serious threat to human health. In recent years, the antibiotic resistance of Salmonella has become serious as well, which has strengthened the harm of Salmonella to human health. In this paper, a simple and effective electrochemical approach was developed to obtain profile of the antimicrobial susceptibility. Screen-printed carbon electrodes (SPCEs) were used to detect various concentrations of target bacteria at the same time, which have good stability, low cost, and easy mass production. The electroactive redox, resazurin, was used to monitor levels of metabolically active bacteria. As a demonstration, the antibacterial effect of ofloxacin and penicillin on Salmonella gallinarum (S. gallinarum) isolates was evaluated, and the minimum inhibitory concentration (MIC) was measured as well. In the real sample measurement, the MIC obtained was similar to that obtained by conventional antimicrobial susceptibility testing (AST). In this assay, bacterial activity was quantified sensitively and accurately, and the detection time was greatly reduced compared with conventional AST (16–20 h), which means that metabolic capacity of live bacteria could be observed after 1 h of incubation. We were able to clearly detect bacteria above 10² CFU/m. This method aims to nonspecific and can be widely applied to the detection of a variety of drug-resistant bacteria, providing an experimental basis for the rational use of antibiotics and bacterial resistance mechanisms.

沙门氏菌是最常见的食源性病原体之一，对人类健康构成了严重威胁。近年来，沙门氏菌的抗生素耐药性也变得越来越严重，从而加强了沙门氏菌对人体健康的危害。在本文中，开发了一种简单有效的电化学方法来获得抗菌药敏性的概况。丝网印刷碳电极（SPCE）用于同时检测各种浓度的靶细菌，具有良好的稳定性，低成本和易于批量生产的特点。电活性氧化还原，刃天青，用于监测代谢活性细菌的水平。作为证明，氧氟沙星和青霉素对鸡沙门氏菌的抗菌作用（评价了S. gallinarum（S. gallinarum）分离株，并测量了最小抑菌浓度（MIC）。在实际样品测量中，获得的MIC与通过常规抗菌药敏测试（AST）获得的MIC相似。在该测定中，灵敏而准确地定量了细菌活性，并且与传统的AST（16-20小时）相比，检测时间大大缩短，这意味着孵育1小时后即可观察到活细菌的代谢能力。我们能够清楚地检测出10 ²  CFU / m以上的细菌。该方法针对非特异性，可广泛应用于多种耐药菌的检测，为合理使用抗生素和耐药机制提供实验依据。