Ca²⁺ is a key element in the sperm activation process of Salmo salar. However, the molecular mechanisms by which this ion enters the sperm cell have been poorly studied. In this study, we examined, for the first time, the role of the voltage-gated T-type Ca²⁺ channel in the activation of sperm motility of Salmo salar. Using an in vitro inhibition assay, a significant decrease in total and progressive motility (P < 0.0001) was observed in Salmo salar sperm when they were treated with NNC-55-0396, a highly selective blocker. The in silico analysis showed that this blocker is docked with a strong affinity for the pore of the voltage-gated T-type calcium channel suggesting the blocking of Ca²⁺ ions. The results show that the T-type voltage-gated Ca²⁺ channel is key to sperm motility in Salmo salar.

Ca ²⁺是Salmo salar精子激活过程中的关键元素。但是，对该离子进入精子细胞的分子机制的研究很少。在这项研究中，我们首次检查了电压门控T型Ca ²⁺通道在Salmo salar精子活力激活中的作用。使用体外抑制试验， 在Salmo ar鱼中观察到总和进行性运动显着降低（P <0.0001）用高度选择性的阻断剂NNC-55-0396治疗精子。电脑分析表明，该阻滞剂与电压门控T型钙通道的孔具有很强的亲和力，表明对Ca ²⁺离子的阻滞。结果表明，T型电压门控的Ca ²⁺通道是Salmo salar精子运动的关键。