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Evaluation of the relationship between DNA methylation status and heterosis in soybean with MethylRAD technique
Euphytica ( IF 1.9 ) Pub Date : 2020-06-01 , DOI: 10.1007/s10681-020-02639-1
Baixing Song , Yuanqian Wang , Songnan Yang , Xueying Li , Xiao Han , Sujie Fan , Abraham Lamboro , Dan Yao , Jing Qu , Zhuo Zhang , Yang Song , Jun Zhang

Heterosis plays an important role in the breeding of commercial crops, but the molecular mechanism behind this phenomenon is still unclear. To better understand the molecular basis of heterosis in soybean, the correlation between DNA methylation status and heterosis in soybean was tested. From three soybean varieties used as parents, six hybrid combinations were generated. Twelve traits of the nine materials were investigated to study the relationship between DNA methylation level differences and heterosis. In this study, MethylRAD technique was used to evaluate the genomic methylation status among the six reciprocal soybean F1 hybrids and their parents in the immature grains. Results showed that the total site number of methylation and methylation levels of the F1 hybrids with strong heterosis were in a status between the two parents and the ones with weak heterosis were generally below that of two parents. Thus, it was beneficial to promote heterosis when the numbers of methylation sites and methylation levels were between two parents. Among three types of methylation variations, the type that parental demethylated and the progeny methylated (type A) may be relative to improve heterosis of some traits. The type A of methylation variations was significantly positively correlated with the mid-parent heterosis (MPH) of the fat content ( r = 0.98**), the branch number per plant ( r = 0.84), and also was significantly positively correlated with the BPH of the branch number per plant ( r = 0.82*) and the stems thick ( r = 0.81*). These results implied that both the methylation and demethylation in hybrids relative to their parents may control the expression of the genes that were associated with partial phenotypic variation in hybrids.

中文翻译:

甲基RAD技术评价大豆DNA甲基化状态与杂种优势的关系

杂种优势在经济作物育种中发挥着重要作用,但这一现象背后的分子机制尚不清楚。为了更好地了解大豆杂种优势的分子基础,测试了 DNA 甲基化状态与大豆杂种优势之间的相关性。从用作亲本的三个大豆品种中,产生了六个杂种组合。研究了 9 种材料的 12 个性状,以研究 DNA 甲基化水平差异与杂种优势之间的关系。在本研究中,MethylRAD 技术用于评估 6 种互惠大豆 F1 杂种及其亲本在未成熟籽粒中的基因组甲基化状态。结果表明,杂种优势强的F1杂种的甲基化总位点数和甲基化水平处于两个亲本之间,而杂种优势弱的杂种则普遍低于两个亲本。因此,当甲基化位点数和甲基化水平介于两个亲本之间时,有利于促进杂种优势。在三种甲基化变异中,亲本去甲基化的类型和子代甲基化的类型(A型)可能与提高某些性状的杂种优势有关。A型甲基化变异与脂肪含量(r=0.98**)、单株分枝数(r=0.84)的中亲杂种优势(MPH)显着正相关,与每株植物分枝数的 BPH ( r = 0. 82*) 和茎粗 (r = 0.81*)。这些结果暗示杂种相对于其亲本的甲基化和去甲基化可能控制与杂种中部分表型变异相关的基因的表达。
更新日期:2020-06-01
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