During gamete delivery in Arabidopsis thaliana, intercellular communication between the attracted pollen tube and the receptive synergid cell leads to subcellular events in both cells culminating in the rupture of the tip-growing pollen tube and release of the sperm cells to achieve double fertilization. Live imaging of pollen tube reception revealed dynamic subcellular changes that occur in the female synergid cells. Pollen tube arrival triggers the trafficking of NORTIA (NTA) MLO protein from Golgi-associated compartments and the accumulation of endosomes at or near the synergid filiform apparatus, a membrane-rich region that acts as the site of communication between the pollen tube and synergids. Domain swaps and site-directed mutagenesis reveal that NTAs C-terminal cytoplasmic tail with its calmodulin-binding domain influences the subcellular localization and function of NTA in pollen tube reception and that accumulation of NTA at the filiform apparatus is necessary and sufficient for MLO function in pollen tube reception.During gamete delivery in Arabidopsis thaliana, intercellular communication between the attracted pollen tube and the receptive synergid cell leads to subcellular events in both cells culminating in the rupture of the tip-growing pollen tube and release of the sperm cells to achieve double fertilization. Live imaging of pollen tube reception revealed dynamic subcellular changes that occur in the female synergid cells. Pollen tube arrival triggers the trafficking of NORTIA (NTA) MLO protein from Golgi-associated compartments and the accumulation of endosomes at or near the synergid filiform apparatus, a membrane-rich region that acts as the site of communication between the pollen tube and synergids. Domain swaps and site-directed mutagenesis reveal that the NTA C-terminal cytoplasmic tail with its calmodulin-binding domain influences the subcellular localization and function of NTA in pollen tube reception and that accumulation of NTA at the filiform apparatus is necessary and sufficient for MLO function in pollen tube reception.

中文翻译：

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花粉管触发的丝状装置上的NORTIA积累促进拟南芥的受精

在拟南芥配子交付期间，被吸引的花粉管和感受性协同细胞之间的细胞间通讯导致两个细胞中的亚细胞事件，最终导致尖端生长的花粉管破裂并释放精子细胞，从而实现双重受精。花粉管接受的实时成像揭示了女性协同细胞中发生的动态亚细胞变化。花粉管到达会触发高尔基体相关区室中NORTIA（NTA）MLO蛋白的运输，以及内体在增生丝状体设备处或附近的积累，内膜的积累是膜丰富的区域，充当花粉管和增效剂之间的交流部位。域交换和定点诱变表明，具有钙调蛋白结合结构域的NTA C末端胞质尾部会影响花粉管接收中NTA的亚细胞定位和功能，而丝状体中NTA的积累对于MLO功能的发挥是必要和充分的。花粉管的接收在拟南芥配子传递过程中，被吸引的花粉管与感受性协同细胞之间的细胞间通讯导致两个细胞发生亚细胞事件，最终导致顶端生长的花粉管破裂并释放出精子细胞，从而达到双倍受精。花粉管接受的实时成像揭示了女性协同细胞中发生的动态亚细胞变化。花粉管到达会触发高尔基体相关区室中NORTIA（NTA）MLO蛋白的运输，并导致内体在协同花丝状设备处或附近积累，内膜的积累是膜丰富的区域，充当花粉管与协同花之间的通讯部位。域交换和定点诱变表明，具有钙调蛋白结合结构域的NTA C末端胞质尾部影响花粉管接收中NTA的亚细胞定位和功能，丝状体中NTA的积累对于MLO功能是必要且充分的在花粉管接待处。