ABSTRACT Increasing the level of AsA and maintaining proper lignin content will improve the quality of celery (Apium graveolens L.). However, the study on the regulation mechanism of genes related to AsA and lignin metabolism in celery was limited, especially in yellow celery. In this study, the accumulation of AsA and lignin and the expression profiles of related-genes were detected in three kinds of yellow celery. The results showed that the content of AsA was higher in the leaf blades of ‘Huangtaiji’ and the petioles of ‘Jinhuangqincai’. The content of lignin was the highest in ‘Jinhuangqincai’ leaves, followed by ‘Liuhehuangxinqin’ and ‘Huangtaiji’. The expression levels of AgAO (r = −0.032) and AgDHAR1 (r = −0.193) genes involved in AsA recycling and degradation showed negatively correlated with AsA content. AgCCoAOMT (r = 0.692) and AgF5 H (r = 0.733) genes expression were positively correlated with lignin content, while the expression of AgCAD (r = −0.694) and AgC3ʹH (r = −0.722) genes were negatively correlated with lignin content. Our current work provided potential theoretical basis for effectively changing accumulation of AsA and lignin by genetic engineering in celery.

中文翻译：

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黄芹中抗坏血酸和木质素的积累及抗坏血酸和木质素相关基因的差异表达

摘要 提高 AsA 水平并保持适当的木质素含量将提高芹菜 (Apium Graveolens L.) 的品质。然而，关于芹菜中AsA和木质素代谢相关基因调控机制的研究还很有限，尤其是在黄芹中。本研究检测了三种黄芹菜中AsA和木质素的积累情况以及相关基因的表达谱。结果表明，'黄太极'叶片和'金黄沁菜'叶柄中AsA含量较高。'金黄沁菜'叶中木质素含量最高，其次是'六合黄心芹'和'黄太极'。参与 AsA 回收和降解的 AgAO (r = -0.032) 和 AgDHAR1 (r = -0.193) 基因的表达水平与 AsA 含量呈负相关。AgCCoAOMT (r = 0。692) 和 AgF5 H (r = 0.733) 基因的表达与木质素含量呈正相关，而 AgCAD (r = -0.694) 和 AgC3ʹH (r = -0.722) 基因的表达与木质素含量呈负相关。我们目前的工作为通过基因工程有效改变芹菜中AsA和木质素的积累提供了潜在的理论基础。