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Diversity of Transmission Outcomes Following Co-Infection of Sheep with Strains of Bluetongue Virus Serotype 1 and 8.
Microorganisms ( IF 4.5 ) Pub Date : 2020-06-05 , DOI: 10.3390/microorganisms8060851
Eva Veronesi 1, 2 , Karin Darpel 1 , Simon Gubbins 1 , Carrie Batten 1 , Kyriaki Nomikou 1, 3 , Peter Mertens 1, 3 , Simon Carpenter 1
Affiliation  

Bluetongue virus (BTV) causes an economically important disease, bluetongue (BT), in susceptible ruminants and is transmitted primarily by species of Culicoides biting midges (Diptera: Ceratopogonidae). Since 2006, northern Europe has experienced multiple incursions of BTV through a variety of routes of entry, including major outbreaks of strains of BTV serotype 8 (BTV-8) and BTV serotype 1 (BTV-1), which overlapped in distribution within southern Europe. In this paper, we examined the variation in response to coinfection with strains of BTV-1 and BTV-8 using an in vivo transmission model involving Culicoides sonorensis, low passage virus strains, and sheep sourced in the United Kingdom. In the study, four sheep were simultaneously infected using BTV-8 and BTV-1 intrathoracically inoculated C. sonorensis and co-infections of all sheep with both strains were established. However, there were significant variations in both the initiation and peak levels of virus RNA detected throughout the experiment, as well as in the infection rates in the C. sonorensis that were blood-fed on experimentally infected sheep at peak viremia. This is discussed in relation to the potential for reassortment between these strains in the field and the policy implications for detection of BTV strains.

中文翻译:

绵羊与蓝舌病毒血清型 1 和 8 菌株共同感染后传播结果的多样性。

蓝舌病毒(BTV)导致经济上重要的疾病,蓝舌病(BT),在易感反刍动物,并且通过的物种首传库蠓蠓(双翅目:蠓科)。自 2006 年以来,北欧经历了通过各种进入途径的多次 BTV 入侵,包括 BTV 血清型 8 (BTV-8) 和 BTV 血清型 1 (BTV-1) 菌株的主要爆发,这两种菌株在南欧分布重叠. 在本文中,我们使用涉及Culicoides sonorensis 的体内传播模型检查了对 BTV-1 和 BTV-8 菌株共感染的反应变化、低传代病毒株和来自英国的绵羊。在该研究中,使用 BTV-8 和 BTV-1 胸腔内接种的C.sonorensis同时感染了四只绵羊,并确定了所有绵羊与这两种菌株的共感染。然而,在整个实验过程中检测到的病毒 RNA 的起始水平和峰值水平,以及在病毒血症高峰时用血液喂养实验感染的绵羊的C.sonorensis的感染率都存在显着差异。讨论了这些菌株在田间重新分类的可能性以及对 BTV 菌株检测的政策影响。
更新日期:2020-06-05
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