Most studies regarding plasma biomedicine applications mainly focus on the oxidative and/or nitrative stress on bacteria, cancer cells, and other treatment objects. In this study, we evaluate the protective effect of appropriate atmospheric pressure plasma jet (APPJ) treatments on oxygen and glucose deprivation (OGD)‐induced neural cell apoptosis, which is a major pathological process during ischemic stroke, based on the physiological functions of NO. Results show that APPJ treatment reduces the OGD‐induced apoptosis by weakening typical OGD injury consequences including loss of mitochondrial membrane potential, the release of cytochrome c from the mitochondria into the cytoplasm, lower antiapoptotic Bcl‐2 expression, and upregulating the proapoptotic protein Bax. Furthermore, APPJ increased intracellular NO production, which is closely related to the cytoprotective effect of APPJ.

中文翻译：

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大气压等离子体治疗通过靶向线粒体来保护神经细胞免受缺血性卒中相关损伤

关于血浆生物医学应用的大多数研究主要集中于细菌，癌细胞和其他治疗对象的氧化和/或硝化应激。在这项研究中，我们根据NO的生理功能，评估适当的大气压等离子体射流（APPJ）处理对氧和葡萄糖剥夺（OGD）诱导的神经细胞凋亡的保护作用，这是缺血性中风期间的主要病理过程。 。结果表明，APPJ治疗通过减弱典型的OGD损伤后果（包括线粒体膜电位丧失，细胞色素c的释放）来减少OGD诱导的凋亡。从线粒体到细胞质，降低抗凋亡Bcl-2的表达，并上调凋亡蛋白Bax。此外，APPJ增加了细胞内NO的产生，这与APPJ的细胞保护作用密切相关。