In this study, we aimed to investigate the presence of asprosin (ASP) in the liver, kidneys, heart, stomach, testicles and brain and to determine the serum and tissue asprosin levels in diabetic rats. A total of 14 male Wistar Albino rats were divided into two groups, each containing 7 rats: (I) control group and (II) experimental diabetes group. Control rats received no treatment and the rats in the experiment group received single-dose of streptozotocin (STZ) (50 mg/kg) dissolved in 0.1 M sodium citrate buffer (pH: 4.5) intraperitoneally. Serum levels of asprosin were measured using ELISA method. The presence of asprosin in hepatic, renal, cardiac, gastric, testicular and brain tissues was investigated using immunohistochemical staining. Asprosin was detected in hepatocytes in the liver, cortical distal tubule cells in the kidney, cardiomyocytes in heart, surface epithelial cells of stomach fundus, interstitial Leydig cells in testes and cortical neurons of the brain. Compared to control group, it was found that diabetic rats had decreased asprosin levels in liver, kidney and heart tissues, increased levels in gastric and testicular tissues and no significant changes in brain tissue. Serum asprosin levels of diabetic rats were found to be decreased compared to the control group.

This is the first study in the literature that reports the presence of asprosin in liver, kidney, heart, stomach, testis and brain tissues in rats. The aim of the study is to determine the presence of ASP, a newly discovered adipokine, in various tissues and to examine tissue and serum level changes in STZ-induced diabetes.

在这项研究中，我们旨在调查肝脏，肾脏，心脏，胃，睾丸和大脑中存在的asprosin（ASP），并确定糖尿病大鼠的血清和组织asprosin水平。将总共​​14只雄性Wistar Albino大鼠分成两组，每组包含7只大鼠：（I）对照组和（II）实验性糖尿病组。对照大鼠未接受治疗，实验组大鼠腹膜内接受单剂量的链脲佐菌素（STZ）（50 mg / kg）溶于0.1 M柠檬酸钠缓冲液（pH：4.5）中。使用ELISA法测量血清中的asprosin水平。使用免疫组织化学染色研究了肝，肾，心脏，胃，睾丸和脑组织中Asprosin的存在。在肝脏的肝细胞，肾脏的远端皮质小管细胞中检测到了Asprosin，心脏中的心肌细胞，胃底的表面上皮细胞，睾丸的间质Leydig细胞和大脑的皮质神经元。与对照组相比，发现糖尿病大鼠肝脏，肾脏和心脏组织中的proprosin水平降低，胃和睾丸组织中的proprosin水平升高，脑组织中无明显变化。与对照组相比，发现糖尿病大鼠的血清血浆prosprosin水平降低。

这是文献中首次报道大鼠肝脏，肾脏，心脏，胃，睾丸和脑组织中存在Asprosin的研究。该研究的目的是确定各种组织中是否存在ASP（一种新发现的脂肪因子），并检查STZ诱导的糖尿病中组织和血清水平的变化。