当前位置: X-MOL 学术Phytochem. Lett. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Identification of Pathways and Genes Associated with Cold and Hot Properties of Chinese Materia Medica Based on Bioinformatics Analysis
Phytochemistry Letters ( IF 1.7 ) Pub Date : 2020-06-05 , DOI: 10.1016/j.phytol.2020.05.012
Yue Wang , Lu Zhou , Shujing Zhang , Zhenzhu Liu , Caiyan Li , Yushan Gao , Yan Sun , Fengjie Zheng , Yuhang Li

The Chinese Materia Medica can be divided into four classes: cold, cool, warm, and hot, which mainly exhibits two opposite properties: cold and hot. This study aimed to explore the scientific essence involved in the cold and hot features of Chinese Materia Medica. The microarray data of twelve cold/hot herbs with typical cold and hot properties (six for each group) were analyzed on the Gene-Cloud of Biotechnology Information. First, we compared the differences in total gene expression profiles and identified differentially expressed genes (DEGs) between two classes of herbs. Subsequently, gene ontology (GO) enrichment analysis, pathway analysis, and gene signal network analysis were performed to screen out related pathways and core genes. This study reveals that the upregulated genes in cold herbs are downregulated in hot herbs, and the genes downregulated in cold herbs were upregulated in hot herbs. The gene expression of the two types of herbs has apparent differences, and the DEGs enrich in six systems, including metabolism, immune system, nervous system, endocrine system, cell proliferation and apoptosis, and hemorheology. PRKACA, PRKACB, PRKCA, ADCY7, PIK3R1, and MAPK1 are the core genes. These results elucidated the objectivity of the differences between cold and hot herbs at a genetic level, and identified related pathways and core genes, which may be a feasible approach for solving the qualitative and even quantitative problems of the cold and hot properties of herbs.



中文翻译:

基于生物信息学分析的中药冷热特性途径和基因鉴定

中药可分为冷,凉,暖和热四类,主要表现出两种相反的性质:冷和热。本研究旨在探讨涉及中药冷热特征的科学本质。在生物技术信息的基因云中分析了十二种具有典型冷热特性的冷热草药的微阵列数据(每组六种)。首先,我们比较了总基因表达谱的差异,并鉴定了两类草药之间的差异表达基因(DEG)。随后,进行了基因本体(GO)富集分析,途径分析和基因信号网络分析,以筛选出相关途径和核心基因。这项研究表明,冷草药中的上调基因在热草药中下调,冷草药中下调的基因在热草药中上调。两种草药的基因表达存在明显差异,DEGs丰富了六个系统,包括新陈代谢,免疫系统,神经系统,内分泌系统,细胞增殖和凋亡以及血液流变学。PRKACA,PRKACB,PRCAA,ADCY7,PIK3R1和MAPK1是核心基因。这些结果阐明了在遗传水平上冷热草药之间差异的客观性,并确定了相关的途径和核心基因,这可能是解决草药冷热性质的定性甚至定量问题的可行方法。神经系统,内分泌系统,细胞增殖和凋亡以及血液流变学。PRKACA,PRKACB,PRCAA,ADCY7,PIK3R1和MAPK1是核心基因。这些结果阐明了在遗传水平上冷热草药之间差异的客观性,并确定了相关的途径和核心基因,这可能是解决草药冷热性质的定性甚至定量问题的可行方法。神经系统,内分泌系统,细胞增殖和凋亡以及血液流变学。PRKACA,PRKACB,PRCAA,ADCY7,PIK3R1和MAPK1是核心基因。这些结果阐明了在遗传水平上冷热草药之间差异的客观性,并确定了相关的途径和核心基因,这可能是解决草药冷热性质的定性甚至定量问题的可行方法。

更新日期:2020-06-05
down
wechat
bug