International Journal of Food Microbiology ( IF 5.4 ) Pub Date : 2020-06-05 , DOI: 10.1016/j.ijfoodmicro.2020.108716 P Khademi 1 , A Ownagh 1 , B Ataei 2 , A Kazemnia 1 , A Enferadi 1 , M Khalili 3 , K Mardani 4
Q fever is a common zoonotic disease with worldwide distribution. The causative agent of Q fever is Coxiella burnetii, a gram-negative and polymorphic rod bacterium. Sheep and goats are the primary reservoirs of this disease, although a variety of animal species can be infected. The main route of Q fever transmission from animals to humans is the inhalation of contaminated aerosols with C. burnetii. The bacterium is excreted in milk of infected animals and therefore; the consumption of unpasteurized milk and dairy products might be a route of coxiella burnetii transmission from animals to humans. The present study was conducted to determine the prevalence of C. burnetii in milk samples collected from sheep and goats in west Azerbaijan province, Iran. During 2018, a total number of 420 milk samples were collected from sheep (n = 210) and goats (n = 210) of different regions of the province. All milk samples were subjected to DNA extraction and examined by a highly and specific nested-PCR method. The results showed that 51 (12.1%) (95% CI: 9.3%–15.6%) examined samples [sheep; n = 16 (7.6%) and goat; n = 35 (16.6%)] were positive for C. burnetii. The prevalence of C. burnetii in goat milk samples was significantly higher than sheep milk samples (P < 0.05). The shedding of C. burnetii in milk was significantly higher in summer (25%) (P < 0.05, 95% CI: 17.7%–34%) than the other seasons. It was concluded that sheep and goat populations in west Azerbaijan play an important role in the epidemiology of Q fever.
中文翻译:
伊朗西北部绵羊和山羊奶中伯氏梭状芽胞杆菌DNA的患病率。
Q热是一种常见的人畜共患病,分布在世界各地。Q热的病原体是克氏杆菌,革兰氏阴性多态杆状细菌。绵羊和山羊是该疾病的主要宿主,尽管可以感染多种动物。Q热从动物传播到人的主要途径是吸入被伯氏梭菌污染的气溶胶。这种细菌从感染动物的牛奶中排泄,因此;未经巴氏消毒的牛奶和奶制品的消费可能是柯氏杆菌从动物向人类传播的一种途径。进行本研究,以确定伯氏梭菌的患病率伊朗阿塞拜疆西部省的绵羊和山羊的牛奶样本中的糖。2018年期间,从 该省不同地区的绵羊(n = 210)和山羊(n = 210)总共收集了420个牛奶样品。所有牛奶样品均经过DNA提取,并通过高度特异性的巢式PCR方法进行检查。结果表明,有51(12.1%)(95%CI:9.3%–15.6%)的样品进行了检测[绵羊;n = 16(7.6%)和山羊;n = 35(16.6%)]对伯氏梭菌呈阳性。山羊奶样品中伯氏梭菌的患病率明显高于羊奶样品(P <0.05)。伯氏梭菌的脱落夏季牛奶的摄入量显着高于其他季节(25%)(P <0.05,95%CI:17.7%–34%)。结论是,阿塞拜疆西部的绵羊和山羊种群在Q热流行病学中起重要作用。