Salivary gland transcriptome analysis of the Asiatic Triatoma rubrofasciata was performed by high-throughput RNA sequencing. This analysis showed that the majority of reads accounting for 85.38% FPKM (fragments per kilobase of exon per million mapped fragments) were mapped with a secreted class. Of these, the most abundant subclass accounting for 89.27% FPKM was the lipocalin family. In the lipocalin family, the most dominant molecules making up 70.49% FPKM were homologues of procalin, a major allergen identified from T. protracta saliva, suggesting an important role in blood-sucking of T. rubrofasciata. Other lipocalins showed similarities to pallidipin and triplatin, inhibitors of collagen-induced platelet aggregation identified from T. pallidipennis and T. infestans, respectively, Td38 from T. dimidiata with unknown function, triatin-like lipocalin with unknown function, and triafestin, an inhibitor of the activation of the kallikrein–kinin system, identified from T. infestans saliva. Other than lipocalin family proteins, homologues of antigen-5 (3.38% FPKM), Kazal-type serine protease inhibitor (1.36% FPKM), inositol polyphosphate 5-phosphatase (1.32% FPKM), and apyrase/5’-nucleotidase (0.64% FPKM) were identified as abundant molecules in T. rubrofasciata saliva. Through this study, de novo assembly of 42,580,822 trimmed reads generated 35,781 trinity transcripts, and a total of 1,272 coding sequences for the secreted class were deposited in GenBank. The results provide further insights into the evolution of salivary components in blood-sucking arthropods.

通过高通量RNA测序对亚洲三叶红藻的唾液腺转录组进行分析。该分析表明，占85.38％FPKM（每百万个外显子每千碱基外显子的片段）的大部分读物都以一个分泌类定位。其中，脂蛋白家族是最丰富的亚类，占FPKM的89.27％。在lipocalin家族中，占70.49％FPKM的最主要分子是procalin的同源物，procalin是从T. protracta唾液中鉴定出的主要过敏原，表明在rub.farofasciata的吸血中起着重要作用。其他脂钙蛋白显示与pallidipin和triplatin相似，后者是从胶原蛋白中鉴定出的胶原蛋白诱导的血小板聚集抑制剂T. pallidipennis和T. infestans分别来自功能不明的T. dimidiata的Td38，功能未知的triatin-like lipocalin和triafestin，其是从T. infestans唾液中鉴定的激肽释放酶-激肽系统活化的抑制剂。除lipocalin家族蛋白外，抗原5（3.38％FPKM），Kazal型丝氨酸蛋白酶抑制剂（1.36％FPKM），肌醇多磷酸5-磷酸酶（1.32％FPKM）和腺苷三磷酸/ 5'-核苷酸酶（0.64％）的同源物FPKM）被鉴定为红腹水T唾液中的丰富分子。通过这项研究，从头开始装配了42,580,822个修剪的读段，产生了35,781个三位一体的转录本，并且共有1,272个针对该分泌类的编码序列存放在GenBank中。结果为吸血节肢动物唾液成分的演变提供了进一步的见解。