The present study, for the first time, assessed the influence of light intensity on morphogenesis of callus cultures and accumulation of β-sitosterol, a key phytosterol compound in date palm. High frequencies of calli with maximum biomass were induced from shoot tip explants in Murashige and Skoog basal medium supplemented with 10 mg/l 2,4 dichlorophenoxyacetic acid + 3 mg/l 2-isopentyladenine. Such proliferating callus cultures of date palm cv. Hayani were incubated in specified medium under different light intensities [0, 14, and 42 µmol/m²/s photosynthetic photon flux density (PPFD)]. The collected data exhibited an increase in callus volume, globularization, newly differentiated somatic embryos, degree of browning, and β-sitosterol contents of callus cultures. Various effects of different light intensities on the above-mentioned traits were recorded. Morphogenesis of callus under dark condition (0 µmol/m²/s PPFD) resulted in a higher degree of globularization and produced maximum number of newly differentiated somatic embryos. Whereas, under gradually increasing light intensities (14 and 42 µmol/m²/s PPFD), a surge in the accumulation of β-sitosterol content in the proliferating callus was observed. Thus, from the present study, it can be deduced that a proper application of suitable light intensity on proliferating callus has beneficial influence on morphogenesis and a higher production of β-sitosterol in date palm.

本研究首次评估了光强度对愈伤组织培养物形态形成和β-谷甾醇（枣树中的关键植物甾醇化合物）积累的影响。在补充10 mg / l 2,4二氯苯氧基乙酸+ 3 mg / l 2-异戊基腺嘌呤的Murashige和Skoog基础培养基中，从茎尖外植体诱导出具有最大生物量的高频率愈伤组织。椰枣的这种愈伤组织培养物。Hayani在指定的培养基中以不同的光强度[0、14和42 µmol / m ²孵育/ s光合光子通量密度（PPFD）]。收集的数据显示出愈伤组织培养物中愈伤组织体积，球状化，新分化的体细胞胚，褐变程度和β-谷固醇含量增加。记录了不同光强度对上述性状的各种影响。在黑暗条件下（0 µmol / m ² / s PPFD）愈伤组织的形态发生导致更高的球状化程度，并产生了新分化体细胞胚的最大数量。而在逐渐增加的光强度下（14和42 µmol / m ²/ s PPFD），观察到增殖的愈伤组织中β-谷甾醇含量的累积激增。因此，从本研究中可以推论，在增殖的愈伤组织上适当地施加适当的光强度对枣树中的形态发生和β-谷甾醇的更高产量具有有益的影响。