Measuring behavior in the form of numerical data is difficult, especially for studies involving complex actions. DanioVision is a closed-chamber system that utilizes subject tracking to comprehensively record behavior, while also mitigating the influence of environmental conditions. We used DanioVision to record activity of juvenile dwarf cuttlefish (Sepia bandensis) during the inaccessible prey (IP) procedure, a memory experiment in which cuttlefish learn to inhibit capture attempts towards inaccessible prey. By quantifying total movement and orientation of the body, we found that cuttlefish show memory by selectively inhibiting tentacle strikes without reducing total movement, or orientation towards the prey. We show that DanioVision can be used to assess multiple components of dynamic responses that are not measurable by direct observation alone and provide new evidence that strike inhibition is the product of learning, and not motor fatigue.

以数字数据形式测量行为是困难的，尤其是对于涉及复杂动作的研究。DanioVision是一种封闭室系统，它利用对象跟踪功能来全面记录行为，同时还减轻了环境条件的影响。我们使用DanioVision少年侏儒乌贼的记录活动（乌贼bandensis）在无法进入的猎物（IP）程序中，进行一项记忆实验，墨鱼学会抑制捕获到无法进入的猎物的企图。通过量化身体的整体运动和方向，我们发现墨鱼通过选择性地抑制触手触击而不降低整体运动或朝向猎物的方向来显示记忆。我们证明DanioVision可用于评估动态反应的多个组成部分，这些组成部分不能通过直接观察来单独测量，并且提供了新的证据表明罢工抑制是学习而非运动疲劳的产物。