当前位置: X-MOL 学术J. Gen. Plant Pathol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
PCR primers to identify and detect Berkeleyomyces rouxiae , a causal agent of black root rot of lettuce
Journal of General Plant Pathology ( IF 1.2 ) Pub Date : 2020-06-05 , DOI: 10.1007/s10327-020-00933-3
Rei Nakane , Toshiyuki Usami

Berkeleyomyces rouxiae, a causal agent of black root rot of lettuce, is morphologically and physiologically indistinguishable from the closely related species B. basicola. PCR primers specific for these species were designed based on the nucleotide sequence of genes specific to the species. The primer pair specific to B. rouxiae did not amplify DNA of other plant pathogens that invade root or stem base of lettuce and was useful for diagnosis and quantitating the pathogen from lettuce root tissue.

中文翻译:

PCR引物,用于鉴定和检测生菜黑根腐病的致病因子rouxiae

生菜黑根腐病的致病因子rouxiae,在形态和生理学上与密切相关的物种B. basicola没有区别。基于对这些物种特异的基因的核苷酸序列设计对这些物种特异的PCR引物。肉豆芽孢杆菌特异的引物对不能扩增入侵莴苣根或茎基的其他植物病原体的DNA,可用于诊断和定量莴苣根组织中的病原体。
更新日期:2020-06-05
down
wechat
bug