The effect of inhibition of the tumor suppressor p53 on the antioxidant system genes expression under the influence of cytotoxic compounds of the platinum group was studied. It was found that the action of platinum(II) and platinum(IV) complexes induced accumulation of p53 protein with a maximum in 12 h, which was confirmed by an increase in the expression of the P21 gene, the target gene of the p53 protein. It was shown that the action of platinum complexes activated the expression of catalase and superoxide dismutase 2 genes. Suppression of p53 protein functions with specific inhibitor α-piphitrin under the action of platinum complexes reduced the expression of catalase and superoxide dismutase 2 genes and the target gene P21, which attested to the p53-dependent regulation of these genes.

中文翻译：

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抑癌基因p53功能对细胞毒性化合物作用下抗氧化系统基因表达的影响

研究了在铂族细胞毒性化合物的影响下，抑癌基因p53对抗氧化系统基因表达的抑制作用。发现铂(II)和铂(IV)配合物的作用诱导p53蛋白的积累在12小时内达到最大值，这通过p53蛋白的靶基因P21基因的表达增加得到证实。 . 结果表明，铂配合物的作用激活了过氧化氢酶和超氧化物歧化酶 2 基因的表达。在铂配合物的作用下，用特异性抑制剂 α-piphitrin 抑制 p53 蛋白功能降低了过氧化氢酶和超氧化物歧化酶 2 基因以及靶基因 P21 的表达，这证明了这些基因的 p53 依赖性调节。