Mycoviruses are widely distributed in a variety of fungal species. However, few viruses have been reported in basal fungi. A novel non-segmented dsRNA virus was isolated from the basal fungus Conidiobolus sp. of the phylum Zoopagomycota, which has been named "Conidiobolus non-segmented RNA virus 1" (CNRV1). The complete genome sequence of CNRV1 was determined by dsRNA extraction, next-generation sequencing, and RACE. The genome of CNRV1 dsRNA is 3,092 bp in length and contains two open reading frames (ORFs) predicted to encode a subgenomic protein 1 (sgP1) and a putative RNA-dependent RNA polymerase (RdRp). Multiple sequence alignment and phylogenetic analysis based on RdRp sequences from selected dsRNA viruses showed that CNRV1 shared 31.9% sequence identity with Nigrospora oryzae unassigned RNA virus 1 (NoNRV1) and clustered with NoNRV1 and four other mycoviruses. These viruses are unassigned and distant from members of the family Partitiviridae, although they were previously considered partitivirus-like viruses. Thus, CNRV1 is a novel member of proposed genus "Unirnavirus", and is the first dsRNA sequence reported from a member of the phylum Zoopagomycota. This study extends our knowledge about mycoviruses in basal fungi.

霉菌病毒广泛分布在各种真菌物种中。但是，基本真菌中几乎没有病毒报道。从基底真菌分生孢子中分离出一种新型的非节段dsRNA病毒sp。Zoopagomycota门的名称，已被命名为“ Conidiobolus非分段RNA病毒1”（CNRV1）。CNRV1的完整基因组序列是通过dsRNA提取，下一代测序和RACE确定的。CNRV1 dsRNA的基因组长度为3,092 bp，包含两个开放阅读框（ORF），预计可编码亚基因组蛋白1（sgP1）和推定的RNA依赖性RNA聚合酶（RdRp）。基于来自选定的dsRNA病毒的RdRp序列的多重序列比对和系统发育分析表明，CNRV1与米曲霉未分配的RNA病毒1（NoNRV1）共享31.9％的序列同一性，并与NoNRV1和其他四种霉菌病毒聚集在一起。这些病毒是未分配病毒，与Partitiviridae家族的成员距离较远，尽管它们以前被视为类似partitivirus的病毒。因此，CNRV1是提出的“ Unirnavirus ”属的新成员，并且是从动物门菌门的成员报道的第一个dsRNA序列。这项研究扩展了我们对基础真菌中的分枝杆菌病毒的认识。