当前位置: X-MOL 学术Arch. Virol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Molecular characterization of hepatitis B virus isolated from Black South African cancer patients, with and without hepatocellular carcinoma.
Archives of Virology ( IF 2.7 ) Pub Date : 2020-06-05 , DOI: 10.1007/s00705-020-04686-4
Daniel Mak 1 , Anna Kramvis 1
Affiliation  

In South Africa (SA), hepatitis B virus (HBV) infection is strongly associated with hepatocellular carcinoma (HCC). As HBV genotypes/subgenotypes and mutations can influence disease manifestation and progression, our aim was to molecularly characterize HBV in Black cancer patients, with and without HCC. The basal core promoter/precore (BCP/PC) and complete surface (S) regions of HBV isolates were amplified and sequenced from 55 HCC cases and 22 non-HCC cancer controls. Phylogenetic analysis of 43 polymerase/complete S region amplicons showed that the majority (88.4%) clustered with subgenotype A1, 4.7% with A2, and 7% with A3. The following mutations were significantly more frequent in HCC cases than in controls (p < 0.05): in the BCP/PC 1753C/G (22.5% vs. 0%), 1764A (69.4% vs. 38.1%), and T64C (51.5% vs. 20%) in the preS2, which results in a F22L substitution. PreS1 and preS2 start codon mutants were detected only in HCC cases, occurring in two and 16 isolates, respectively. PreS deletion mutants were isolated from 11 HCC cases, which had a HBV viral load > 10,000 IU/mL and were significantly younger than non-HCC controls (34 ± 7.1 vs. 41.2 ± 9.5 years, p = 0.05). The 1762T/1764A double mutation was detected in the majority (90.9%) of the isolates from HCC cases with preS deletions. Black HBV carriers were mainly infected with subgenotype A1, with HCC cases carrying BCP/PC and preS mutant strains that are associated with hepatocarcinogenesis. This is the first study to compare the molecular characteristics of HBV from HCC and non-HCC cancer patients in SA.



中文翻译:

分离自黑人南非癌症患者的乙型肝炎病毒的分子特征,有无肝细胞癌。

在南非(SA),乙肝病毒(HBV)感染与肝细胞癌(HCC)密切相关。由于HBV基因型/亚基因型和突变会影响疾病的表现和进展,因此我们的目标是对患有或不患有HCC的黑人癌症患者的HBV进行分子表征。从55例HCC病例和22例非HCC癌症对照中扩增并测序了HBV分离株的基础核心启动子/前核心(BCP / PC)和完整表面(S)区。对43个聚合酶/完整S区扩增子的系统进化分析表明,大多数(88.4%)与亚基因型A1聚在一起,4.7%与A2和7%与A3聚在一起。在HCC病例中,以下突变的发生率明显高于对照组(p<0.05):在preS2中的BCP / PC 1753C / G(22.5%vs. 0%),1764A(69.4%vs.38.1%)和T64C(51.5%vs 20%)中,导致F22L替代。仅在HCC病例中检测到PreS1和preS2起始密码子突变体,分别出现在两个和16个分离物中。从11例HCC病例中分离出PreS缺失突变体,这些病例的HBV病毒载量> 10,000 IU / mL,并且比非HCC对照年轻得多(34±7.1 vs. 41.2±9.5岁,p= 0.05)。在大多数具有preS缺失的HCC分离株中,检测到1762T / 1764A双重突变(90.9%)。黑色HBV携带者主要感染亚型A1,HCC病例携带BCP / PC和与肝癌发生有关的preS突变株。这是首次比较SA中HCC和非HCC癌症患者的HBV分子特征的研究。

更新日期:2020-06-05
down
wechat
bug