Dinitolmide is a nitro amide coccidiostat used in poultry feed, and is a potential threat to the environment and human health. In this study, a monoclonal antibody (mAb) against dinitolmide was prepared and an immunochromatographic assay (ICA) was developed to detect residual dinitolmide in chicken tissue. The results show that the mAb exhibited high sensitivity, with a limit of detection as low as 9.01 ng mL⁻¹. A cross reactivity test revealed that the mAb also had good specificity for dinitolmide. This ICA method showed a visible limit of detection of 2.5 μg kg⁻¹, and a cut-off value of 25 μg kg⁻¹ for testing dinitolmide in chicken sample extract by using the naked eye. Importantly, these observations using our ICA, were comparable to other methods of detection such as liquid chromatography, tandem mass spectrometry and indirect competitive enzyme-linked immunosorbent assays (IC-ELISA). These data suggest that our ICA method is a reliable, portable, and high-throughput method for the detection of dinitolmide residues in chicken tissue.

中文翻译：

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基于金纳米颗粒的侧向流动带的开发，用于检测鸡肉组织中的迪诺米特。

Dinitolmide是一种用于禽类饲料的硝基酰胺类抗球虫药，对环境和人类健康构成潜在威胁。在这项研究中，制备了针对狄诺糖胺的单克隆抗体（mAb），并开发了一种免疫色谱法（ICA）以检测鸡组织中残留的狄诺糖胺。结果显示，mAb表现出高灵敏度，检测限低至9.01 ng mL ^-1。交叉反应性测试表明，mAb对狄诺糖胺也具有良好的特异性。此方法ICA表明检测2.5微克公斤的可见极限^-1，和25的截止值微克千克^-1用肉眼检测鸡肉样品提取物中的狄诺米特。重要的是，使用我们的ICA进行的观察与其他检测方法（例如液相色谱，串联质谱和间接竞争性酶联免疫吸附测定（IC-ELISA））可比。这些数据表明，我们的ICA方法是一种可靠，便携式且高通量的方法，可检测鸡肉组织中的狄诺糖胺残留。