The macrolactam antibiotic incednine, isolated from Streptomyces sp. ML694–90F3, contains a (S)-3-aminobutyric acid moiety in its polyketide aglycon. In this study, we performed mutasynthesis to generate incednine derivatives. We successfully obtained 28-methylincednine by feeding 3-aminopentanoic acid into culture of a strain in which the glutamate 2,3-aminomutase gene idnL4, whose product is responsible for supplying 3-aminobutyric acid, was disrupted. 28-Methylincednine showed similar suppressive activity of the antiapoptotic function of oncoprotein Bcl-xL to that of incednine. Thus, this study highlights the applicability of the mutasynthesis approach in generation of novel β-amino acid-containing macrolactam polyketide derivatives.

从内生链霉菌中分离出的大环内酰胺类抗菌素。ML694–90F3在其聚酮化合物糖苷配基中包含（S）-3-氨基丁酸部分。在这项研究中，我们进行了突变合成，以生成手工艺衍生物。我们成功地通过将3-氨基戊酸送入一个菌株的培养物中成功获得了28-甲基茚二碱，在该菌株中，负责提供3-氨基丁酸的谷氨酸2，3-氨基变位酶基因idnL4被破坏。28-甲基亚铁亚胺对癌蛋白Bcl-xL的抗凋亡功能的抑制活性与因西得宁相似。因此，本研究强调了诱变方法在产生新型含β-氨基酸的大内酰胺聚酮化合物衍生物中的适用性。