Abstract

The species Annona nutans (R. E. Fries) is a plant found in Bolivia, Paraguay, Argentina and the Brazilian cerrado. Considering the anti-inflammatory and antinociceptive activities of the hydrometanolic fraction (FHMeOH) of A. nutans leaves previously reported, the present study aimed to evaluate in vivo anti-inflammatory and antinociceptive activities of a subfraction obtained from FHMeOH, the butanolic fraction (FBuOH_f). Intraperitoneal (i.p.) treatment with FBuOH_f (50 and 100 mg · kg⁻¹) inhibited paw edema induced by carrageenan. Moreover, FBuOH_f (100 mg · kg⁻¹, i.p.) also suppressed polymorphonuclear (PMN) leukocyte migration in the footpad. Regarding the antinociceptive activity, FBuOH_f (50, 100, and 200 mg · kg⁻¹, i.p.) inhibited acetic acid-induced abdominal writhing. In the formalin test, this fraction (200 mg · kg⁻¹, i.p.) reduced licking time only in the inflammatory phase. The FBuOH_f contents flavonoids and cinnamic acid derivatives, such as quercetin-3-O-galactoside, quercetin-3-O-glucoside, isorhamnetin-3-O-galactoside, quercetin-3-O-β-D-apio-furanosyl-(1→2)-galactopyranoside and chlorogenic acid, identified and quantified by LC-MS. The FBuOH_f possesses anti-inflammatory and peripheral antinociceptive activities.

摘要

该品种番荔枝碱草（RE薯条）在玻利维亚，巴拉圭，阿根廷和巴西的热带草原发现的植物。考虑到先前报道的A. nutans叶子的氢甲醇组分 (FHMeOH) 的抗炎和镇痛活性，本研究旨在评估从 FHMeOH 获得的亚组分的体内抗炎和镇痛活性，丁醇组分 (FBuOH _f）。用FBuOH _f (50和100mg·kg ^-1 )腹膜内(ip)处理抑制由角叉菜胶诱导的爪水肿。此外，FBuOH _f (100 mg·kg ^-1, ip) 还抑制了足垫中的多形核 (PMN) 白细胞迁移。关于镇痛活性，FBuOH _f (50, 100, 和 200 mg·kg ^-1 , ip) 抑制乙酸引起的腹部扭动。在福尔马林试验中，该部分（200 mg·kg ^-1，ip）仅在炎症期减少舔舐时间。所述FBuOH _˚F内容黄酮和肉桂酸衍生物，如槲皮素-3- ø半乳糖苷，栎精-3- ø葡糖苷，异鼠李素-3- ø半乳糖苷，栎精-3- O-β -D- APIO-furanosyl- (1→2)-吡喃半乳糖苷和绿原酸，通过 LC-MS 进行鉴定和定量。FBuOH _f 具有抗炎和外周镇痛活性。