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Inactive dimeric structure of the protease domain of stomatin operon partner protein.
Acta Crystallographica Section D ( IF 2.2 ) Pub Date : 2020-06-04 , DOI: 10.1107/s2059798320005021
Hideshi Yokoyama 1 , Kana Suzuki 2 , Kodai Hara 2 , Ikuo Matsui 3 , Hiroshi Hashimoto 2
Affiliation  

The N‐terminal region of the stomatin operon partner protein (STOPP) PH1510 (1510‐N) from the hyperthermophilic archaeon Pyrococcus horikoshii is a serine protease with a catalytic Ser–Lys dyad (Ser97 and Lys138) and specifically cleaves the C‐terminal hydrophobic region of the p‐stomatin PH1511. In a form of human hemolytic anemia known as hereditary stomatocytosis, stomatin is deficient in the erythrocyte membrane owing to mis‐trafficking. Stomatin is thought to act as an oligomeric scaffolding protein to support cell membranes. The cleavage of stomatin by STOPP might be involved in a regulatory system. Several crystal structures of 1510‐N have previously been determined: the wild type, the K138A mutant and its complex with a substrate peptide. Here, the crystal structure of the S97A mutant of 1510‐N (1510‐N S97A) was determined at 2.25 Å resolution. The structure contained two 1510‐N S97A molecules in the asymmetric unit. On the superposition of one monomer of the 1510‐N S97A and wild‐type dimers, the S97A Cα atom of the other monomer of 1510‐N S97A deviated by 23 Å from that of the wild type. This result indicates that 1510‐N can greatly change the form of its dimer. Because of crystallographic symmetry in space group P65, a sixfold helical structure is constructed using the 1510‐N dimer as a basic unit. This helical structure may be common to STOPP structures.

中文翻译:

胃抑素操纵子伴侣蛋白的蛋白酶结构域的非活性二聚体结构。

嗜热古球菌火球菌(Pyrococcus horikoshii)的Stomatin操纵子伴侣蛋白(STOPP)PH1510(1510-N)的N端区域是一种丝氨酸蛋白酶,带有催化的Ser-Lys二聚体(Ser97和Lys138),并特异性切割p-stomatin PH1511的C端疏水区域。人为溶血性贫血是一种遗传性气孔增多症,由于人口贩运,stomatin在红细胞膜中缺乏。Stomatin被认为是支持细胞膜的寡聚支架蛋白。STOPP对胃抑素的切割可能与调节系统有关。先前已经确定了1510-N的几种晶体结构:野生型,K138A突变体及其与底物肽的复合物。在这里,以2.25Å的分辨率确定了1510-N(1510-N S97A)的S97A突变体的晶体结构。该结构在不对称单元中包含两个1510-N S97A分子。1510-N S97A的其他单体的α原子与野生型的原子偏离23Å。该结果表明1510-N可以极大地改变其二聚体的形式。由于空间群P 6 5中的晶体对称性,使用1510-N二聚体作为基本单位构建了六重螺旋结构。这种螺旋结构可能是STOPP结构所共有的。
更新日期:2020-06-04
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