The effects of hepatocyte nuclear factors (HNFs) have been established in various tumors; however, the roles of HNF‐1β in colorectal cancer progression are never been found. In the present study, HNF‐1β expression was initially detected in clinical tissue samples and online datasets and HNF‐1β was found to be highly expressed in colorectal cancer tissues. In addition, a positive correlation existed between HNF‐1β expression and the overall survival of patients with colorectal cancer. In vitro and in vivo experiments revealed that HNF‐1β suppressed the stemness and migration of colorectal cancer cells. Combined with microRNAs (miRNAs) based on transcriptome‐sequencing analysis, mechanistic studies showed that HNF‐1β directly bound to miR‐200b promoter and thus promoted miR‐200b expression, this HNF‐1β/miR‐200b resulted in the downregulation of the expression of miR‐200b downstream effectors. Furthermore, HNF‐1β inhibits the stemness and migration of colorectal cancer cells through miR‐200b. This study reveals a novel HNF‐1β/miR‐200b axis responsible for the stemness of colorectal cancer cells.

中文翻译：

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肝细胞核因子-1β通过促进miR-200b活性抑制大肠癌细胞的干性和迁移。

肝细胞核因子（HNF）的作用已在各种肿瘤中确立。然而，尚未发现HNF-1β在大肠癌进展中的作用。在本研究中，最初在临床组织样本和在线数据集中检测到HNF-1β表达，并且发现HNF-1β在结直肠癌组织中高表达。此外，HNF-1β表达与结直肠癌患者的整体生存之间存在正相关。体外和体内实验表明，HNF-1β抑制了结直肠癌细胞的干性和迁移。机理研究表明，结合基于转录组测序分析的microRNA（miRNA），HNF-1β直接与miR-200b启动子结合，从而促进了miR-200b的表达，该HNF-1β/ miR-200b导致miR-200b下游效应子表达下调。此外，HNF-1β通过miR-200b抑制结直肠癌细胞的干性和迁移。这项研究揭示了负责结直肠癌细胞干性的新型HNF-1β/ miR-200b轴。