High levels of gums such as dextran, produced by Leuconostoc and Lactobacillus spp., have a severe impact on factory throughput and sugar quality. This study aimed to determine the phylogenetic relationships between gum‐producing Leuconostoc and Lactobacillus bacteria which were isolated from various locations in a sugarcane processing factory at times when low‐ and high‐dextran raw sugar, respectively, were produced. Phylogenetic analysis of 16S rRNA gene sequences grouped 81 isolates with the type strains of Leuconostoc mesenteroides (subspp. mesenteroides, dextranicum, and cremoris), Leuconostoc pseudomesenteroides, Leuconostoc lactis, and Leuconostoc citreum, respectively. Forty‐three isolates clustered with the type strain of Lactobacillus fermentum. The phylogenetic relatedness of the isolates was determined by sequencing and analysis of the housekeeping genes rpoA and dnaA for Leuconostoc spp. and the pheS and tuf genes for the Lactobacillus spp. The rpoA gene proved discriminatory for the phylogenetic resolution of all of the isolated Leuconostoc spp. and the dnaA housekeeping gene was shown to be effective for isolates clustering with the type strains of Leuc. mesenteroides and Leuc. citreum. None of the loci examined permitted differentiation at the subspecies level of Leuc. mesenteroides. Single‐locus analysis, as well as the concatenation of the pheS and tuf housekeeping gene sequences, yielded identical phylogenies for the Lactobacillus isolates corresponding to L. fermentum.

中文翻译：

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从甘蔗加工流中分离出的亮豆和乳杆菌种的系统发育分析。

Leuconostoc和Lactobacillus spp。生产的高含量的口香糖，如右旋糖酐，对工厂的生产量和糖品质有严重影响。这项研究旨在确定在分别生产低葡聚糖和高葡聚糖原糖时，从甘蔗加工厂的不同地点分离的产胶的古柯诺菌和乳杆菌细菌之间的系统发育关系。16S rRNA基因序列的系统发育分析分组81个分离株的型菌株明串珠菌（subspp。肠系膜，dextranicum，和乳脂），假菜青虾，乳酸扁豆虾和柠檬扁豆虾。有43株与发酵乳杆菌型菌株聚集在一起的菌株。分离株的系统发育相关性是通过对Leuconostoc spp的管家基因rpoA和dnaA进行测序和分析来确定的。以及乳杆菌属的pheS和tuf基因。该RPOA基因证明歧视的所有隔离的亲缘分辨率明串珠菌。和dnaA已显示持家基因对于与Leuc型菌株聚集的分离株有效。mesenteroides和Leuc。明串珠菌。没有一个检查的基因座允许在Leuc的亚种水平上分化。肠系膜。单基因座分析以及pheS和tuf看家基因序列的串联，产生了与发酵乳杆菌相对应的乳杆菌分离株的相同系统发育。