The aim of the present study was to prepare and characterize nanocomposite films to improve the treatment of skin wounds by applying the film as a bandage. To modify chitosan (Cs) and to prepare nanocomposites, a mixture between titanium dioxide nanoparticles (TiO₂NPs) was performed at different concentrations (2, 5, 10 and 15 wt%) and oleic acid (OA). The thin nanocomposite films were prepared by using casting method. The prepared films (Cs, Cs/TiO₂NPs, Cs/OA and Cs/OA/TiO₂NPs) were described by water absorption (swelling study) and biological degradation. Physico‐chemical characterizations of Cs, Cs/OA, Cs/TiO₂NPs and Cs/OA/TiO₂NPs (with only 15 wt% TiO₂NPs) films were determined by X‐ray diffraction, transmission high‐resolution electron microscopy, field emission scanning electron microscopy, thermal analysis and Fourier transform infrared spectroscopy as well as their mechanical properties. Antimicrobial activity against microorganisms has been studied to assess activity against bacteria. The prepared nanocomposite films showed good antimicrobial activity for both Gram‐positive and Gram‐negative bacteria. The therapeutic effects of Cs‐TiO₂NPs‐oleic acid nanocomposites on healing excision wounds were studied in rat animal model. The data obtained revealed that groups treated with nanocomposites showed enhancement wound closure and speed up wound healing time.

中文翻译：

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基于油酸改性壳聚糖/TiO2纳米颗粒聚合物薄膜的生物纳米复合材料的生物学应用研究。

本研究的目的是制备和表征纳米复合薄膜，以通过将薄膜用作绷带来改善皮肤伤口的治疗。为了改性壳聚糖 (Cs) 并制备纳米复合材料，在不同浓度（2、5、10 和 15 wt%）和油酸 (OA) 下进行二氧化钛纳米颗粒 (TiO ₂ NPs)的混合。采用流延法制备纳米复合薄膜。通过吸水（溶胀研究）和生物降解描述了制备的薄膜（Cs、Cs/TiO ₂ NPs、Cs/OA 和 Cs/OA/TiO ₂ NPs）。Cs、Cs/OA、Cs/TiO ₂ NPs 和 Cs/OA/TiO ₂ NPs（只有 15 wt% TiO ₂通过 X 射线衍射、透射高分辨率电子显微镜、场发射扫描电子显微镜、热分析和傅里叶变换红外光谱以及它们的机械性能来测定 NPs) 薄膜。已经研究了针对微生物的抗微生物活性以评估针对细菌的活性。制备的纳米复合薄膜对革兰氏阳性菌和革兰氏阴性菌均显示出良好的抗菌活性。在大鼠动物模型中研究了Cs-TiO ₂ NPs-油酸纳米复合材料对愈合切除伤口的治疗作用。获得的数据显示，用纳米复合材料处理的组显示出增强的伤口闭合和加速伤口愈合时间。