Thermosulfuriphilus ammonigenes ST65^T is an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent chimney. T. ammonigenes is an obligate chemolithoautotroph utilizing elemental sulfur as an electron donor and nitrate as an electron acceptor with sulfate and ammonium formation. It also is able to grow by disproportionation of elemental sulfur, thiosulfate and sulfite. Here, we present the complete genome sequence of strain ST65^T. The genome consists of a single chromosome of 2,287,345 base pairs in size and has a G + C content of 51.9 mol%. The genome encodes 2172 proteins, 48 tRNA genes, and 3 rRNA genes. Genome analysis revealed a complete set of genes essential to CO₂ fixation and gluconeogenesis. Homologs of genes encoding known enzyme systems for nitrate ammonification are absent in the genome of T. ammonigenes assuming unique mechanism for this pathway. The genome of strain ST65^T encodes a complete set of genes necessary for dissimilatory sulfate reduction, which are probably involved in sulfur disproportionation and anaerobic oxidation. This is the first reported genome of a bacterium from the genus Thermosulfuriphilus, providing insights into the microbial contribution into carbon, sulfur and nitrogen cycles in the deep-sea hydrothermal vent environment.

产氨热嗜盐菌ST65 ^T是从深海热液喷口烟囱中分离出来的厌氧嗜热细菌。产氨链球菌是专性化自养生物，利用元素硫作为电子供体，硝酸盐作为电子受体形成硫酸盐和铵盐。它也能够通过元素硫，硫代硫酸盐和亚硫酸盐的歧化来生长。在这里，我们介绍了菌株ST65 ^T的完整基因组序列。该基因组由一个大小为2287345个碱基对的染色体组成，G + C含量为51.9 mol％。基因组编码2172个蛋白质，48个tRNA基因和3个rRNA基因。基因组分析揭示了一整套对CO ₂至关重要的基因固定和糖异生。假定该途径具有独特的机制，则氨曲霉基因组中不存在编码用于硝酸盐氨化的已知酶系统的基因的同源物。ST65 ^T菌株的基因组编码了异化硫酸盐还原所必需的完整基因集，这些基因可能与硫歧化和厌氧氧化有关。这是首次报道的嗜热嗜热菌属细菌的基因组，提供了对深海热液喷口环境中微生物对碳，硫和氮循环的贡献的见解。