Abstract As a biocompatible natural polymer material, chitosan (CS) is widely used in biomedical fields due to its excellent properties such as antibacterial ability. However, the main drawback of chitosan is that it cannot dissolve in water, seriously limiting its application in biomedical field. In this study, CS was successfully modified with a zwitterionic 2-(methyl acryloyoxy) ethyl choline phosphate (MCP) monomer by Michael addition reaction to obtain choline phosphate functionalized chitosan (CS-MCP). The CS-MCP exhibited an excellent water solubility. The antibacterial activity of CS-MCP was tested against S. aureus and E. coli in vitro, the results indicated that the antibacterial property of CP-MCP dissolved in water was as well as that of CS dissolved in acetic acid. The studies of cytotoxicity and hemolysis of CS-MCP showed that CS-MCP had excellent cytocompatibility and low hemolysis ratio. Thus, the water soluble chitosan derivative CS-MCP with good antibacterial ability and biocompatibility has great potential in biomedicine.

中文翻译：

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具有抗菌性能和优异水溶性的两性离子磷酸胆碱功能化壳聚糖

摘要 壳聚糖（CS）作为一种生物相容性天然高分子材料，以其优异的抗菌能力等特性被广泛应用于生物医学领域。然而，壳聚糖的主要缺点是不溶于水，严重限制了其在生物医学领域的应用。在这项研究中，通过迈克尔加成反应用两性离子2-（甲基丙烯酰氧基）乙基胆碱磷酸胆碱（MCP）单体成功修饰CS，获得磷酸胆碱功能化壳聚糖（CS-MCP）。CS-MCP 表现出优异的水溶性。体外对CS-MCP对金黄色葡萄球菌和大肠杆菌的抗菌活性进行了测试，结果表明CP-MCP溶于水的抗菌性能与溶于乙酸的CS一样。CS-MCP的细胞毒性和溶血研究表明，CS-MCP具有良好的细胞相容性和较低的溶血率。因此，具有良好抗菌能力和生物相容性的水溶性壳聚糖衍生物CS-MCP在生物医学方面具有巨大的潜力。