Purpose

To investigate the interaction of a novel low molecular weight hyaluronic acid derivative containing hydrophobic groups with soft contact lenses and its effect on lens hydrophilicity compared with a conventional form of hyaluronic acid.

Methods

This investigation studied the uptake of fluorescently-labelled hyaluronic acid and a low molecular weight hyaluronic acid derivative to four types of contact lenses using fluorescent microscopy and confocal laser scanning microscopy. Further, the four lens types were used to compare efficacy in improving hydrophilicity, as well as maintenance of contact angle measurements, in commercially available multipurpose solutions that contained either hyaluronic acid, the low molecular weight hyaluronic acid derivative, or an alternative wetting agent.

Results

The low molecular weight hyaluronic acid derivative was found to sorb more readily to silicone hydrogel lenses and exhibit a greater accumulation over time than conventional hyaluronic acid. Multipurpose solutions containing the low molecular weight hyaluronic acid derivative showed an increase in lens hydrophilicity through decreases in contact angle measurements when compared with those obtained from lenses treated with multipurpose solutions containing conventional hyaluronic acid or alternative wetting agents. This increase in lens hydrophilicity associated with the low molecular weight hyaluronic acid derivative was also maintained over multiple cycles in phosphate buffered saline, while alternative solutions with conventional hyaluronic acid did not.

Conclusion

Overall, lens treatment using a low molecular weight hyaluronic acid derivative-based solution lead to improved in vitro lens hydrophilicity.

中文翻译：

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低分子量透明质酸衍生物对隐形眼镜润湿性的影响

目的

研究含有疏水基团的新型低分子量透明质酸衍生物与软性隐形眼镜的相互作用及其与传统形式的透明质酸相比对镜片亲水性的影响。

方法

本研究使用荧光显微镜和共聚焦激光扫描显微镜研究了四种类型的隐形眼镜对荧光标记的透明质酸和低分子量透明质酸衍生物的吸收。此外，四种镜片类型用于比较在含有透明质酸、低分子量透明质酸衍生物或替代润湿剂的市售多用途溶液中提高亲水性以及保持接触角测量的功效。

结果

发现低分子量透明质酸衍生物比常规透明质酸更容易吸附到硅酮水凝胶镜片上并且随着时间的推移表现出更大的积累。与用含有常规透明质酸或替代润湿剂的多用途溶液处理的镜片相比，含有低分子量透明质酸衍生物的多用途溶液通过接触角测量值的降低显示出镜片亲水性的增加。这种与低分子量透明质酸衍生物相关的镜片亲水性增加也在磷酸盐缓冲盐水中保持了多个循环，而具有常规透明质酸的替代溶液则没有。

结论

总体而言，使用基于低分子量透明质酸衍生物的溶液进行镜片处理可改善体外镜片亲水性。