Winter survival develops efficient tolerance mechanisms in plants by regulating cold-responsive and cold-regulated genes at the transcriptional level. Hence, an insight into the expression would provide the molecular function of these cold responsive genes. In this study, an uncharacterized gene encoding a cold-regulated (Cor413) protein identified from Saccharum spontaneum (wild relative species of sugarcane) low-temperature transcriptome with environmental adaptability is isolated and characterized. The full-length coding region possesses an open reading frame of 642 bp, which encodes a putative polypeptide of 213 amino acids of molecular weight 25.6 kDa and an isoelectric point (Pi) of 9.69. The SsCor413 sequence showed a high similarity to monocot Cor413 proteins comprising a WCOR413 domain. Bioinformatics analysis revealed that Cor413 protein has multispanning transmembrane helices along with highly conserved phosphorylation sites. String analysis suggested that SsCor413 is grouped with LEA and Rab proteins that are involved in freezing tolerance. Gene ontology analysis assigned the protein to terms such as “plasma membrane,” “cold acclimation,” and “response to cold.” Sub-cellular localization experiments of sugarcane callus and onion epidermal cells indicated the nuclear localized expression. Quantitative gene expression analysis indicated that the SsCor413 gene is up-regulated in leaf and root tissues of S. spontaneum under low temperature, salinity, and water deficit stress conditions. These results highlight the potential role of SsCor413 in abiotic stress tolerance, and this gene could be a new candidate for combating multiple stresses in sugarcane.

中文翻译：

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来自自发糖的核局部非生物胁迫响应冷调节基因 413 (SsCor413) 的分离和表征

冬季生存通过在转录水平调节冷响应和冷调节基因在植物中形成有效的耐受机制。因此，深入了解表达将提供这些冷响应基因的分子功能。在这项研究中，从具有环境适应性的 Saccharum spontaneum（甘蔗的野生亲缘种）低温转录组中鉴定出的编码冷调节 (Cor413) 蛋白的未表征基因被分离和表征。全长编码区具有 642 bp 的开放阅读框，编码 213 个氨基酸的推定多肽，分子量为 25.6 kDa，等电点 (Pi) 为 9.69。SsCor413 序列显示出与包含 WCOR413 域的单子叶植物 Cor413 蛋白的高度相似性。生物信息学分析表明，Cor413 蛋白具有多跨膜螺旋和高度保守的磷酸化位点。字符串分析表明 SsCor413 与参与冷冻耐受性的 LEA 和 Rab 蛋白归为一组。基因本体分析将蛋白质分配给诸如“质膜”、“冷适应”和“对寒冷的反应”等术语。甘蔗愈伤组织和洋葱表皮细胞的亚细胞定位实验表明核定位表达。定量基因表达分析表明，SsCor413 基因在低温、盐度和水分亏缺胁迫条件下在自发植物的叶和根组织中上调。这些结果突出了 SsCor413 在非生物胁迫耐受中的潜在作用，