To study the effect of manganese exposure on the herbivorous insect Lymantria dispar asiatica, fourth-instar larvae were fed a MnCl₂-amended diet (LdMn) for 84 h (0.40 mmol MnCl₂/g diet). Larvae were weighed before and after the diet administration to assess larval gain in mass under manganese exposure. The whole bodies of half of the survivors were ground in liquid nitrogen for measuring enzyme activities and total antioxidant capacity (T-AOC). The intestinal tracts of the remaining survivors were collected and immediately frozen in liquid nitrogen for 16S rDNA sequencing of gut microbiota. Larvae under manganese stress lost significant mass (p < 0.05). The activities of digestive and antioxidant enzymes and T-AOC, but not trehalase and polyphenol oxidase, were significantly higher after Mn exposure, (p < 0.05). A Venn diagram illustrated that the gut microbial OTU composition in the larvae also changed. Community pies and correlation heatmaps also showed different relative abundances of gut microbes. In other words, species quantity and relative abundance of gut microbes agreed with PCoA visualization and indicated that the gut microbial community in L. dispar asiatica larvae differed significantly between control and LdMn. Functional classification also suggested that exposure to manganese stress significantly decreased gut microbial coenzyme transport and metabolism in L. dispar asiatica larvae. These results further our understanding about stress response of L. dispar asiatica larvae.

为了研究锰暴露对食草昆虫天蛾（Lymantria dispar asiatica）的影响，向四龄幼虫喂饲MnCl ₂改良饲料（LdMn）84小时（0.40 mmol MnCl ₂ / g饲料）。在饮食管理前后对幼虫称重，以评估在锰暴露下幼虫的质量增加。将一半幸存者的整个身体在液氮中研磨，以测量酶活性和总抗氧化能力（T-AOC）。收集其余幸存者的肠道，并立即在液氮中冷冻，用于肠道菌群的16S rDNA测序。锰胁迫下的幼虫失去了明显的质量（p <0.05）。锰暴露后，消化酶和抗氧化酶和T-AOC的活性显着升高，而海藻糖酶和多酚氧化酶则没有（P  <0.05）。维恩图说明幼虫中肠道微生物OTU的组成也发生了变化。社区饼图和相关热图还显示出肠道微生物的相对相对丰度不同。换句话说，肠道微生物的种类数量和相对丰度与PCoA可视化相吻合，这表明对照和LdMn之间，积雪草幼虫的肠道微生物群落存在显着差异。功能分类还表明，锰胁迫显着降低了亚洲L. dispar asiatica的肠道微生物辅酶转运和代谢幼虫。这些结果使我们进一步了解了亚洲L. dispar asiatica幼虫的应激反应。