Macroalgae are considered to be one of the rich lignocellulosic biomass materials. Aquatic biomass has gained more attention to biofuels generation in recent years due to its renewable, abundant, and environmentally friendly aspects. Macroalgae are photosynthetic organisms that are found in both marine and freshwater environments. These are considered as a third-generation feedstock for the production of biofuels since they have the ability to synthesize a high amount of lipids, proteins, and carbohydrates. This research study aimed to evaluate the potential of bioethanol production from macroalgae (Rhizoclonium sp.) biomass. The fermentation process was applied in the research by two-way separate hydrolysis and fermentation (SHF). Algae biomass undergoes a pretreatment process to release necessary sugars for yeast digestion. The fermentation process was carried at 30 to 35 °C in the incubator. Finally, the percentage of ethanol was estimated by the ebulliometer. Fermentation was enhanced by immobilization of yeast, which showed the highest concentration of ethanol (65.43 ± 18.13 g/l) after 96 h of fermentation and can be reused for several times for fermentation. Moreover, these study results confirmed that freshwater macroalgae biomass is a suitable and susceptible raw material for bioethanol production.

大型藻被认为是丰富的木质纤维素生物质材料之一。近年来，由于水生物质具有可再生，丰富和环境友好的特点，因此越来越引起人们对生物燃料生产的关注。大型藻是在海洋和淡水环境中都发现的光合生物。由于它们具有合成大量脂质，蛋白质和碳水化合物的能力，因此被视为生产生物燃料的第三代原料。这项研究旨在评估从大型藻类（根瘤菌属）生产生物乙醇的潜力sp。）生物质。通过双向分离水解和发酵（SHF）技术将发酵过程应用于研究。藻类生物质经过预处理过程以释放酵母消化所需的糖。发酵过程在培养箱中于30到35 ° C进行。最后，通过浊度计估算乙醇的百分比。固定化酵母可增强发酵，发酵96小时后，乙醇显示最高浓度（65.43±18.13 g / l），可重复使用多次以进行发酵。此外，这些研究结果证实，淡水大型藻类生物质是生产生物乙醇的合适且易受影响的原料。