The gene sequence encoding the mannose-binding homodimeric protein Allium sativum leaf agglutinin (ASAL) was introduced into maize inbred lines to achieve resistance against sap-sucking corn leaf aphids in transgenic lines. Maize transformants were generated after the co-cultivation of immature maize embryos with the Agrobacterium strain LB4404 harbouring a recombinant Ti-binary ASAL construct driven by the polyubiquitin promoter. The preliminary screening of maize transformants was performed via GUS histochemical analysis, and PCR and Southern blotting confirmed the insertion of the transgene and its stable integration in five transgenic maize lines. Transcript abundance was quantified by a quantitative real-time PCR assay, which revealed variable expression of the ASAL transgene among five transgenic maize lines. The highest mRNA expression of the ASAL gene was found in the A23 transgenic maize line, while the lowest expression was found in the AU1 transgenic maize line. In planta bioassays in the T₁ progeny of the transgenic maize lines revealed high resistance against corn leaf aphids compared to the control non-transgenic line. The mortality of the infesting aphids (Rhopalosiphum maidi) was found to vary from 40 to 71% compared to that of the non-transgenic control maize line.

将编码甘露糖结合的同型二聚体蛋白小葱叶片凝集素（ASAL）的基因序列引入玉米近交系，以实现对转基因系中吸汁的玉米叶片蚜虫的抗性。将未成熟的玉米胚与农杆菌共培养后产生玉米转化体菌株LB4404具有由多泛素启动子驱动的重组Ti-二元ASAL构建体。通过GUS组织化学分析对玉米转化体进行了初步筛选，PCR和Southern印迹证实了转基因的插入及其在5个转基因玉米品系中的稳定整合。转录本丰度通过实时定量PCR分析定量，该分析揭示了五个转基因玉米品系中ASAL转基因的可变表达。在A23转基因玉米品系中发现了ASAL基因的最高mRNA表达，而在AU1转基因玉米品系中发现了最低的表达。在植物体内进行T ₁生物测定转基因玉米品系的后代与对照非转基因品系相比显示出对玉米叶蚜的高抗性。发现与非转基因对照玉米品系相比，侵染性蚜虫（Rhodolosiphum maidi）的死亡率为40-71 ％。