Abstract A recombinant strain producing a complex of extracellular enzymes including chitinase from Myceliophtora thermophila was created based on the fungus Penicillium verruculosum . The activity of the enzyme preparations obtained from the cultural fluid of the producer strain was 0.55, 0.53, and 0.66 U/mg protein with chitin and chitosans with the molecular weight of 200 and 1000 kDa, respectively. The temperature optimum for the recombinant chitinase was 52-65°C; the pH optimum was 4.5-6.2, which corresponded to the published data for this class of the enzymes. The content of heterologous chitinase in the obtained enzyme preparations was 47% of total protein content in the cultural fluid. Enzyme preparations produced by the recombinant P. verruculosum XT403 strain and containing heterologous chitinase were able to degrade the mycelium of micromycetes, including phytopathogenic ones, and were very efficient in the bioconversion of microbiological industry waste.

中文翻译：

---

用获得的酶制剂产生几丁质酶生产者和破坏微菌细胞壁

摘要 在真菌Penicillium v​​erruculosum 的基础上，从嗜热毁丝菌(Myceliophtora thermophila) 中建立了生产包括几丁质酶在内的胞外酶复合物的重组菌株。从生产菌株的培养液中获得的酶制剂的活性分别为0.55、0.53和0.66 U/mg蛋白质，几丁质和壳聚糖的分子量分别为200和1000 kDa。重组几丁质酶的最适温度为52-65℃；最适 pH 值为 4.5-6.2，这与该类酶的公开数据相符。所得酶制剂中异源几丁质酶的含量为培养液总蛋白含量的47%。重组 P. 生产的酶制剂。