Chloramphenicol (CAP) and cyclo-(l-Val-l-Pro) were previously isolated from Streptomyces sp., SUK 25 which exhibited a high potency against methicillin-resistant Staphylococcus aureus (MRSA). This study aimed to profile gene expression of MRSA treated with CAP and cyclo-(l-Val-l-Pro) compounds using DNA microarray. Treatment of MRSA with CAP resulted in upregulation of genes involved in protein synthesis, suggesting the coping mechanism of MRSA due to the inhibition of protein synthesis effect from CAP. Most upregulated genes in cyclo-(l-Val-l-Pro) were putative genes with unknown functions. Interestingly, genes encoding ribosomal proteins, cell membrane synthesis, DNA metabolism, citric acid cycle and virulence were downregulated in MRSA treated with cyclo-(l-Val-l-Pro) compound, suggesting the efficacy of this compound in targeting multiple biological pathways. Contrary to CAP, with only a single target, cyclo-(l-Val-l-Pro) isolated from this study had multiple antimicrobial targets that can delay antibiotic resistance and hence is a potential antimicrobial agent of MRSA.

中文翻译：

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响应于从链霉菌属中分离的环-(l-Val-l-Pro) 和氯霉素的耐甲氧西林金黄色葡萄球菌的基因表达分析，SUK 25 揭示了多个生物靶点的基因下调

氯霉素 (CAP) 和环-(l-Val-l-Pro) 先前从 Streptomyces sp., SUK 25 中分离出来，其对耐甲氧西林金黄色葡萄球菌 (MRSA) 表现出高效力。本研究旨在使用 DNA 微阵列分析用 CAP 和环-(l-Val-l-Pro) 化合物处理的 MRSA 的基因表达。用 CAP 处理 MRSA 导致参与蛋白质合成的基因上调，表明 MRSA 的应对机制是由于 CAP 对蛋白质合成作用的抑制。环-(l-Val-l-Pro) 中的大多数上调基因是功能未知的推定基因。有趣的是，在用环-(l-Val-l-Pro) 化合物处理的 MRSA 中，编码核糖体蛋白、细胞膜合成、DNA 代谢、柠檬酸循环和毒力的基因被下调，表明该化合物在靶向多种生物途径方面的功效。与 CAP 不同，从这项研究中分离出的环-(l-Val-l-Pro) 只有一个目标，它具有多个可以延迟抗生素耐药性的抗菌目标，因此是 MRSA 的潜在抗菌剂。