Pseudomonas fluorescens has the ability to produce the siderophore pyoverdine, a biotechnologically significant iron chelator, which has a wide range of potential applications, such as in agriculture (iron fertilizers) and medicine (development of antibiotics). The present work aimed to evaluate the influence of culture medium composition on the production of siderophores by P. fluorescens DSM 50090, an industrial relevant strain. It was found that the bacterium grown in minimal medium succinate (MMS) had a higher siderophore production than in King B medium. The replacement of succinate by glycerol or dextrose, in minimal medium, originated lower siderophore production. The increase of succinate concentration, the addition of amino acids or the reduction of phosphate in the culture medium did not improve siderophore production by P. fluorescens. The results obtained strongly suggest that (i) MMS is more appropriate than King B for large-scale production of siderophores; (ii) the modification of the culture medium composition, particularly the type of carbon source, influences the level of siderophore secreted; (iii) the production of siderophore by P. fluorescens seems to be a tightly regulated process; once a maximum siderophore concentration has been reached in the culture medium, the bacterium seems to be unable to produce more compound.

荧光假单胞菌（Pseudomonas fluorescens）具有生产铁载体吡overdine的能力，该铁螯合物是生物技术上重要的铁螯合剂，具有广泛的潜在应用，例如在农业（铁肥）和医药（抗生素开发）中。本工作旨在评估培养基组成对荧光假单胞菌DSM 50090（一种工业相关菌株）生产铁载体的影响。已发现，在基本琥珀酸培养基（MMS）中生长的细菌比在King B培养基中具有更高的铁载体产量。在基本培养基中，用甘油或右旋糖代替琥珀酸酯导致较低的铁载体生成。培养基中琥珀酸盐浓度的增加，氨基酸的添加或磷酸盐的减少并未改善荧光假单胞菌的铁载体产生。获得的结果强烈表明：（i）MMS比King B更适合大规模生产铁载体；（ii）培养基组成的改变，特别是碳源的类型，影响着铁载体的分泌水平；（iii）荧光假单胞菌产生铁载体似乎是一个严格监管的过程；一旦在培养基中达到最大铁载体浓度，细菌似乎无法产生更多的化合物。