Cylindrospermopsin (CYN) is one of the most concerning cyanotoxins due to its potential toxicity and spreading to various environments including drinking water. CYN has potential interferences with human and animal metabolic pathways, which influence the functions of organs including liver, kidneys, lungs, etc. CYN is involved in the inhibition of protein synthesis and detachment of ribosomes from the endoplasmic reticulum membrane. It also interacts with soluble proteins, which are associated with protein translations. It is believed that cytochrome 450 is responsible for the rapid toxicity of CYN. Researchers are urged to develop a high-throughput screening method for the detection of CYN in water. Construction of low cost, rapid, and sensitive analytical methods for the detection of CYN is challenging. Here, we used graphene oxide (GO) as the fluorescence sensing platform for probing the high affinity of the short aptamer derived from the wild-type long aptamer-CYN sensing. The biosensor construction involved two steps: first, quenching the fluorescence of fluorescent-labelled truncated aptamer using GO as a quencher and, second, fluorescence recovery in the presence of CYN by competitive binding between the target and GO. One of the truncate aptamers has a 12-fold higher affinity and enhances sensitivity compared to the long aptamer sequence. The limit of detection of the high affinity truncated aptamer is 17 pM which is 6-fold lower than the long aptamer (100 pM). The sensor specifically detects CYN in the presence of other potential interfering toxins. The performance of the sensor was validated using CYN spiked tap water with very good recovery percentage. A rapid and highly sensitive detection of CYN from water resources has been achieved using this method.

Cylindrospermopsin（CYN）由于其潜在毒性并扩散到包括饮用水在内的各种环境中，因此是最受关注的氰毒素之一。CYN对人和动物的代谢途径有潜在的干扰，影响肝脏，肾脏，肺等器官的功能。CYN参与抑制蛋白质合成和核糖体从内质网膜的分离。它还与可溶性蛋白质相互作用，而可溶性蛋白质与蛋白质翻译有关。相信细胞色素450负责CYN的快速毒性。敦促研究人员开发一种高通量筛选方法以检测水中的CYN。用于检测CYN的低成本，快速，灵敏的分析方法的构建具有挑战性。这里，我们使用氧化石墨烯（GO）作为荧光传感平台，以探测源自野生型长适体-CYN传感的短适体的高亲和力。生物传感器的构建涉及两个步骤：首先，使用GO作为淬灭剂淬灭荧光标记的截短适体的荧光，其次，在CYN存在下通过靶标与GO之间的竞争性结合回收荧光。与长适体序列相比，截短适体之一的亲和力高12倍，并提高了敏感性。高亲和力截短适体的检出限为17 pM，比长适体（100 pM）低6倍。该传感器可在存在其他潜在干扰毒素的情况下特异性检测CYN。使用CYN加标自来水验证了传感器的性能，回收率非常好。使用该方法可以快速，高度灵敏地检测水资源中的CYN。