Purpose

Taenia multiceps resides in the small intestine of carnivores such as dogs, foxes, woles, jackals, while Coenurus cerebralis which is the larval form usually settle in the central nervous system and spinal cord of intermediate hosts like sheep, cattle and goats. The aim of this study was to analyze the haplotype diversity, genetic variation and population structure of the mt-CO1 gene sequences of sheep isolates of T. multiceps had been submitted to GenBank from different countries.

Methods

A total of 102 sequences from the mt-CO1 gene fragment belong to T. multiceps sheep isolates were used for bioinformatic analyses. Haplotype analysis, phylogenetic analysis and diversity, neutrality, fixation and gene flow analyses were applied to the sequences.

Results

As a result, 20 haplotypes together with different multiple nucleotide changes were determined after the sequence analysis. Trimmed fragment length was 337 bp hereby 19 polymorphic areas, 12 of which were parsimony informative, were identified, and any insertion–deletion was found. The number of mutations between major haplotypes and the others range from one to nine. The highest (0.72) genetic differentiation (Fst value) was observed between Turkey and Egypt populations while the lowest (− 0.22) was reported from Greece. These findings are important in terms of showing the diversity of nucleotide variation in T. multiceps sheep isolates.

Conclusions

This study serves as the basis for future large-scale studies on T. multiceps worldwide epidemiology, bioecology, geographic distribution and population structure.

中文翻译：

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多头绦虫线粒体细胞色素 C 氧化酶亚基 1 (CO1) 基因的生物多样性和保护状态的计算机分析。

目的

多头带绦虫存在于狗、狐狸、狼、豺等食肉动物的小肠中，而脑尾带蚴是幼虫形式，通常栖息在绵羊、牛和山羊等中间宿主的中枢神经系统和脊髓中。本研究的目的是分析不同国家提交给GenBank的多头孢霉绵羊分离株mt-CO1基因序列的单倍型多样性、遗传变异和种群结构。

方法

来自 mt-CO1 基因片段的总共 102 个序列属于T. multiceps绵羊分离株用于生物信息学分析。对序列应用单倍型分析、系统发育分析和多样性、中性、固定和基因流分析。

结果

结果，在序列分析后确定了 20 个单倍型以及不同的多核苷酸变化。修剪后的片段长度为 337 bp，因此鉴定了 19 个多态性区域，其中 12 个是简约信息，并且发现了任何插入-缺失。主要单倍型和其他单倍型之间的突变数量从一到九不等。在土耳其和埃及种群之间观察到最高 (0.72) 的遗传分化（Fst 值），而最低 (- 0.22) 来自希腊。这些发现对于显示多头绦虫绵羊分离物中核苷酸变异的多样性很重要。

结论

该研究为未来对多头孢子菌全球流行病学、生物生态学、地理分布和种群结构进行大规模研究奠定了基础。