Aeromonas hydrophila is an important water-borne pathogen that leads to a great economic loss in aquaculture. Along with the abuse of antibiotics, drug-resistant strains rise rapidly. In addition, the biofilms formed by this bacterium limited the antibacterial effect of antibiotics. Bacteriophages have been attracting increasing attention as a potential alternative to antibiotics against bacterial infections. Five phages against pathogenic A. hydrophila, named N21, W3, G65, Y71 and Y81, were isolated. Morphological analysis by transmission electron microscopy revealed that phages N21, W3 and G65 belong to the family Myoviridae, while Y71 and Y81 belong to the Podoviridae. These phages were found to have broad host spectra, short latent periods and normal burst sizes. They were sensitive to high temperature but had a wide adaptability to the pH. In addition, the phages G65 and Y81 showed considerable bacterial killing effect and potential in preventing formation of A. hydrophila biofilm; and the phages G65, W3 and N21 were able to scavenge mature biofilm effectively. Phage treatments applied to the pathogenic A. hydrophila in mice model resulted in a significantly decreased bacterial loads in tissues. Five A. hydrophila phages were isolated with broad host ranges, low latent periods, and wide pH and thermal tolerance. And the phages exhibited varying abilities in controlling A. hydrophila infection. This work presents promising data supporting the future use of phage therapy.

中文翻译：

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抗强毒嗜水气单胞菌的噬菌体的分离和鉴定。

嗜水气单胞菌是重要的水传播病原体，导致水产养殖业的巨大经济损失。随着抗生素的滥用，耐药菌株迅速增加。此外，这种细菌形成的生物膜限制了抗生素的抗菌作用。作为对付细菌感染的抗生素的潜在替代品，噬菌体已经引起了越来越多的关注。分离出五个针对病原性嗜水气单胞菌的噬菌体，分别命名为N21，W3，G65，Y71和Y81。透射电镜的形态学分析表明，噬菌体N21，W3和G65属于肌病毒科，而Y71和Y81属于猫病毒科。发现这些噬菌体具有较宽的宿主谱，较短的潜伏期和正常的爆发大小。它们对高温敏感，但对pH具有广泛的适应性。另外，噬菌体G65和Y81显示出相当大的细菌杀灭作用，并且在防止亲水嗜水杆菌生物膜形成方面具有潜力。噬菌体G65，W3和N21能够有效清除成熟的生物膜。小鼠模型中致病性嗜水性链球菌的噬菌体处理可显着降低组织中的细菌载量。分离出五个嗜水链球菌噬菌体，它们具有较宽的宿主范围，较低的潜伏期以及较宽的pH和耐热性。噬菌体在控制嗜水链球菌感染中表现出不同的能力。这项工作提出了有前途的数据，支持未来噬菌体治疗的应用。W3和N21能够有效清除成熟的生物膜。小鼠模型中致病性嗜水性链球菌的噬菌体处理可显着降低组织中的细菌载量。分离出五个嗜水链球菌噬菌体，它们具有较宽的宿主范围，较低的潜伏期以及较宽的pH和耐热性。噬菌体在控制嗜水链球菌感染中表现出不同的能力。这项工作提出了有前途的数据，支持将来噬菌体治疗的应用。W3和N21能够有效清除成熟的生物膜。小鼠模型中致病性嗜水性链球菌的噬菌体处理可显着降低组织中的细菌载量。分离出五个嗜水链球菌噬菌体，它们具有较宽的宿主范围，较低的潜伏期以及较宽的pH和耐热性。噬菌体在控制嗜水链球菌感染中表现出不同的能力。这项工作提出了有前途的数据，支持将来噬菌体治疗的应用。