Butyric acid is a platform chemical material, the production of which has been greatly stimulated by the diverse range of downstream applications in many industries. In particular, higher quality butyric acid used in food and medicine, is more dependent on microbiological production methods. Hence, the bio-oxidation of butanol to butyric acid has been identified as a promising method with good potential economic and environmental benefits. However, both butanol and butyric acid are usually intensively toxic to most microorganisms as well as the bio-oxidation pathway. To develop a green, efficient and competitive microbiological method is the primary work to overcome the bottleneck of butyric acid industry. A combined bioprocess was designed with alternative whole-cell catalysis for butyric acid bio-conversion from butanol by Gluconobacter oxydans in a sealed-oxygen supply bioreactor (SOS). In the operation system, the escape of volatile substrates and toxic chemicals to cells can be avoided by the use of a sealed bioreactor, combined with the rejuvenation of cells by supplying energy co-factors. Finally, during a one-batch whole-cell catalysis, the utilization rate of substrate increased from 56.6 to 96.0% by the simple skill. Additionally, the techno-practical bioprocess can realize the purpose of cell-recycling technology through the rejuvenation effect of co-factor. Finally, we obtained 135.3 g/L butyric acid and 216.7 g/L sorbose during a 60-h whole-cell catalysis. This techno-practical technology provides a promising approach to promote the industrial production of butyric acid with more competitiveness. The techno-practical biotechnology has powerfully promoted the process of butyric acid production by microorganisms, especially makes up for the lack of aerobic fermentation in the industry, and surmounts the shortcomings of traditional anaerobic fermentation. At the same time, this technically practical system provides a promising approach for the promotion of the industrial production of butyric acid in a more competitive manner.

中文翻译：

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一种克服氧化葡糖杆菌全细胞催化过程中丁醇和丁酸的生物毒性和挥发性障碍的技术实用方法。

丁酸是一种平台化工材料，许多行业下游应用的多样化极大地刺激了丁酸的生产。特别是用于食品和医药的更高质量丁酸，更依赖于微生物生产方法。因此，丁醇生物氧化制丁酸已被认为是一种具有良好潜在经济效益和环境效益的有前景的方法。然而，丁醇和丁酸通常对大多数微生物以及生物氧化途径都具有强烈毒性。开发绿色、高效、有竞争力的微生物方法是克服丁酸产业瓶颈的首要工作。在密封供氧生物反应器 (SOS) 中设计了一种组合生物工艺，该工艺具有替代的全细胞催化，用于通过氧化葡糖杆菌从丁醇生物转化丁酸。在操作系统中，通过使用密封的生物反应器，可以避免挥发性底物和有毒化学物质逃逸到细胞中，并通过提供能量辅助因子使细胞恢复活力。最后，在一批全细胞催化过程中，通过简单的技巧，底物的利用率从56.6%提高到96.0%。此外，技术实用的生物工艺可以通过辅因子的再生作用实现细胞回收技术的目的。最后，我们在 60 小时的全细胞催化过程中获得了 135.3 g/L 的丁酸和 216.7 g/L 的山梨糖。该技术实用技术为促进丁酸工业化生产提供了一条更有竞争力的途径。技术实用的生物技术有力地推动了微生物生产丁酸的过程，特别是弥补了工业上好氧发酵的不足，克服了传统厌氧发酵的不足。同时，该技术实用系统为以更具竞争力的方式促进丁酸的工业化生产提供了一条有前景的途径。克服了传统厌氧发酵的缺点。同时，该技术实用系统为以更具竞争力的方式促进丁酸的工业化生产提供了一条有前景的途径。克服了传统厌氧发酵的缺点。同时，该技术实用系统为以更具竞争力的方式促进丁酸的工业化生产提供了一条有前景的途径。