当前位置: X-MOL 学术Ann. Anim. Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
A new concept in minimally invasive embryo transfer
Annals of Animal Science ( IF 1.9 ) Pub Date : 2020-06-02 , DOI: 10.2478/aoas-2020-0034
Jarosław Wieczorek 1 , Ewa Stodolak-Zych 2 , Krzysztof Okoń 3 , Jurij Koseniuk 4 , Magdalena Bryła 5 , Jacek Jura 5 , Mirosław Cegła 5 , Katarzyna Poniedziałek-Kempny 5 , Iwona Rajska 5
Affiliation  

Abstract Considerable variation in embryo transfer (ET) catheter types, diverging opinions on their quality and functionality, complications following the insertion of catheters, low efficiency of the application of ET methods in humans, and their widely varying efficiency in animals demonstrate the need to improve ET methods and to look for new types of catheters. Such an opportunity is offered by the introduction of catheters made of new-generation biomaterials. This study was aimed to introduce a new generation of biomaterials into reproductive biotechnology. New-generation materials were compared with materials that have been used for many years, and the functionality of newly produced catheters was compared in vivo. Five types of biomaterials were tested: polycaprolactone (PCL), dibutyryl chitin (DBC), polypropylene (PP), polyethylene (PE) and polylactide (PLA). The study was carried out in two stages. Firstly, the basic utility parameters such as geometric stability, surface structure and catheter resistance were evaluated. Subsequently, the biocompatibility of selected biomaterials in embryo cultures was examined, and the development potential of the obtained blastocysts was evaluated. In the second stage, in vivo with live animals, the biomaterials were tested for biocompatibility and the obtained catheters were examined for their ET functionality. Efficiency with the use of the newly produced catheters was determined, the quality of the blastocysts obtained after embryo culture in the uterus was assessed, and oviducts were subjected to histopathological examination after embryo transfer. Of the tested biomaterials, only polyethylene (PE) showed adequate biological and material properties and proved suitable for production of ET catheters.

中文翻译:

微创胚胎移植的新概念

摘要 胚胎移植 (ET) 导管类型的巨大差异、对其质量和功能的不同意见、插入导管后的并发症、ET 方法在人类中的应用效率低下以及它们在动物中的广泛不同的效率表明需要改进ET 方法并寻找新型导管。由新一代生物材料制成的导管的引入提供了这样的机会。本研究旨在将新一代生物材料引入生殖生物技术。将新一代材料与已使用多年的材料进行比较,并在体内比较新生产的导管的功能。测试了五种类型的生物材料:聚己内酯 (PCL)、二丁酰甲壳素 (DBC)、聚丙烯 (PP)、聚乙烯 (PE) 和聚丙交酯 (PLA)。该研究分两个阶段进行。首先,对几何稳定性、表面结构和导管阻力等基本实用参数进行了评估。随后,检查了选定的生物材料在胚胎培养中的生物相容性,并评估了获得的囊胚的发育潜力。在第二阶段,在活体动物体内,测试生物材料的生物相容性,并检查获得的导管的 ET 功能。确定使用新生产的导管的效率,评估子宫内胚胎培养后获得的囊胚的质量,并在胚胎移植后对输卵管进行组织病理学检查。在测试的生物材料中,
更新日期:2020-06-02
down
wechat
bug