Metabolomics can be significantly influenced by a range of pre-analytical factors, such as sample collection, pre-processing, aliquoting, transport, storage and thawing. This therefore shows the crucial need for standardizing the pre-analytical phase with the aim of minimizing the inter-sample variability driven by these technical issues, as well as for maintaining the metabolic integrity of biological samples to ensure that metabolomic profiles are a direct expression of the in vivo biochemical status. This review article provides an updated literature revision of the most important factors related to sample handling and pre-processing that may affect metabolomics results, particularly focusing on the most commonly investigated biofluids in metabolomics, namely blood plasma/serum and urine. Finally, we also provide some general recommendations and best practices aimed to standardize and accurately report all these pre-analytical aspects in metabolomics research.

中文翻译：

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代谢组学中血液和尿液样品的分析前处理标准化的建议和最佳实践。

代谢组学可能会受到一系列分析前因素的显着影响，例如样品收集，预处理，等分试样，运输，储存和解冻。因此，这表明对分析前阶段进行标准化的迫切需要，其目的是最小化这些技术问题驱动的样品间变异性，以及保持生物样品的代谢完整性，以确保代谢组学特征直接表达体内生化状态。这篇评论文章提供了与可能影响代谢组学结果的样品处理和预处理相关的最重要因素的最新文献修订，特别着重于代谢组学中最常研究的生物流体，即血浆/血清和尿液。最后，